GET A QUOTE

Leptin Receptor (LEP-R) ELISA Kit

  • Created on the 7 February, 2017.

BACKGROUND

Leptin receptor (also called LEP-R, obese receptor and OB-R) is a single membrane-spanning receptor which belongs to the class I cytokine receptor family which includes: G-CSF receptor, LIF receptor and IL-6 receptor (gp130). LEP-R has a major role as a receptor that are specific for the fat cell hormone leptin, it is also designated as a cluster of differentiation 295 (CD295). It is primarily produced in adipose tissue and is able to bind leptin which is an approx. 16kDa double protein, this interaction of leptin with its receptor is intimately vital for regulating fat levels within the body. Another important role of leptin is to regulate blood pressure through the leptin receptor.

It is possible for the leptin receptor to have multiple splice forms, however, only the longest splice form is found to have any significant cytosolic domain and this appears sufficient for cytosolic signalling. This splice form has been shown to be over expressed in the hypothalamus and leads to the activation of STAT3 and STAT5. Since, leptin is able to control food intake and energy balance via the leptin receptor in the hypothalamus, this could suggest that many obesity or obesity-related diseases could be the result of some polymorphisms of the leptin receptor gene (LEPR).

INTENDED USE

Human leptin receptor ELISA kit is a reliable quantitative procedure for measuring leptin receptor (LEP-R) in human serum, plasma, cell culture supernatant, tissue lysates and body fluids. This assay has a minimum sensitivity detection limit of < 8.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of leptin receptor (LEP-R) using this leptin receptor ELISA kit was approximately < 8.0 pg/ml. The dynamic assay range for this kit is 156.0 – 10,000.0 pg/ml.

REFERENCES

  1. Hypertension in obesity and the leptin receptor gene locus. J. Clin. Endocr. Metab. (2000) 85: 3126-3131. Rosmond, R., et al.
  2. A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature (1998) 392: 398-401. Clement, K., et al.
  3. Identification and expression cloning of a leptin receptor, OB-R. Cell (1995) 83: 1263-1271. Tartaglia, L. A., et al.
  4. Polymorphisms in the leptin receptor (LEPR)–putative association with obesity and T2DM. J. Hum. Genet. (2006) 51: 85-91. Park, K. S., et al.

ADDITIONAL INFORMATION

  • Full Name: Leptin Receptor (LEP-R) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Body Fluids, Cell Culture Supernatant, Tissue Lysates
  • Sensitivity: < 8.0 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford