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Leptin ELISA Kit

  • Created on the 7 February, 2017.

BACKGROUND

Leptin which is the product of the obese (OB) gene is a single chain of 146 amino acids sequence that is 16kDa in size. It is a circulating hormone which can be found to be abundantly and more specifically expressed in the adipose tissues, playing a vital function in fat metabolism, body weight regulation and appetite control. Leptin is known to target the central nervous system, in particular the hypothalamus where it can affect food intake. It has a dual action of either increasing energy consumption or decreasing the appetite for food, leading to a net effect of more fat being burnt. In humans, the levels of leptin that is present correlate with body mass index (BMI) and the percentage body fat, both of these found to be elevated in obese individuals.

In addition to its role in metabolic control, there is evidence that it can be important in neuroendocrine signalling, reproduction and is a powerful inhibitor of bone formation. There are many other factors which can regulate leptin action apart from the amount of body fat include: glucocorticoids, insulin, sex hormones and catecholamines. The standard product used in this ELISA assay is recombinant human leptin.

INTENDED USE

Human leptin ELISA kit is a reliable quantitative procedure for measuring leptin in human serum, plasma (EDTA, heparin) and cell culture supernatant. This assay has a minimum sensitivity detection limit of < 10.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of leptin using this human leptin ELISA kit was approximately < 10.0 pg/ml. The dynamic assay range for this kit is 62.5 – 4000.0 pg/ml.

REFERENCES

  1. Serum Leptin level is a regulator of bone mass. Proc. Nat. Acad. Sci. (2004) 101: 3258-3263. Elefteriou, F., et al.
  2. Nonadipose tissue production of Leptin: Leptin as a novel placenta-derived hormone in humans. Nature Med. (1997) 3: 1029-1033. Masuzaki, H., et al.
  3. Leptin. Lancet (1998) 351: 737-742. Auwerx, J. and Staels, B.
  4. The human obese (OB) gene: RNA expression pattern and mapping on the physical, cytogenetic, and genetic maps of chromosome 7. Genome Res. (1995) 5: 5-12. Green, E. D., et al.
  5. Human obese gene expression: adipocyte-specific expression and regional differences in the adipose tissue. Diabetes (1995) 44: 855-858. Masuzaki, H., et al.

ADDITIONAL INFORMATION

  • Full Name: Leptin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (Heparin, EDTA), Cell Culture Supernatant
  • Sensitivity: < 10.0 pg/ml

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