Rat/Mouse Leptin ELISA Kit

Full Name: Rat/Mouse Leptin ELISA Kit
Reactivity: Rat, Mouse
Sample Type: Serum, Plasma
Sensitivity: 10.0 pg/ml

BACKGROUND

Adipose tissue fat cells secrete leptin, this is essential for regulating appetite, body weight and food intake. Adipose tissue releases it into the circulation to let the body know when it has enough fat reserves after overeating or being deprived of food. It increases lipolysis, leading to energy release from fat cells as heat (thermogenesis). It also inhibits brown adipose tissue thermogenesis in order to use more energy for maintenance and cell repair. Leptin also decreases fat storage and increases protein breakdown. Concentrations are high before eating, then increase rapidly after a meal and slowly return to basal levels after about 2 hours.

There are many publications which have indicated that resistance to leptin or the absence can lead to uncontrolled feeding and subsequent weight gain. It has been investigated as a novel therapeutic for the treatment of certain diseases including metabolic syndrome. Leptin itself does not affect food intake from one meal to the next but can effectively alter food intake and also control long term energy expenditure. It is released by fat cells that signals the brain to stop eating and start burning stored energy. When fat on your body is burned due to physical activity, leptin levels in your blood increases. When you take in too much food, too much leptin is released, which tells the brain there’s enough energy stored up to avoid starvation and so it should stop feeding — but high levels of leptin can cause feelings of lethargy or malaise.

INTENDED USE

Rat/mouse leptin ELISA kit is a procedure for analysing quantitative amounts of mouse or rat total leptin (obese protein) in serum and plasma. This assay has a minimum sensitivity detection limit of 10.0 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Pre-Coated Plate: Anti-leptin antibody.
Standards (A-G)(Lyophilized): Recombinant, concentrations 25, 50, 100, 200, 400, 800, 1600pg/ml.
Control KS.
Antibody Conjugate.
Enzyme Conjugate.
Dilution Buffer.
Washing Buffer (20x Conc.).
TMB Substrate Solution.
Stopping Solution.
Sealing Tape.

SENSITIVITY

The minimum sensitivity detection limit of mouse and rat total leptin using current rat or mouse leptin ELISA kit was approximately 10.0 pg/ml. The dynamic range for this assay is 25.0 – 1,600 pg/ml.

ASSAY CHARACTERISTICS

– Absorbance values of the blank should be below 0.2, these of standard G (1600 pg/ml) should exeed 1.5.
– Determined and calculated concentration of Control KS should be within the range of the concentration given on vial label (±2SD).
– A higher-grade polynomial, or four parametric logistic (4-PL) curve fit or non-linear regression are usually suitable for the evaluation.

REFERENCES

Circulating leptin levels are modulated by fasting, cold exposure and insulin_administration in lean but not Zucker (fa/fa) rats as measured by ELISA. Biochem Biophys Res Commun. (1996) 223 (3): 660-5. Hardie L.J., et al.
Leptin does not induce hypertrophy, cell cycle alterations, or production of MCP-1 in cultured ratand mouse cardiomyocytes. Endocr Res. (2005) 31 (4): 375-86. Piñieiro R., et al.
Effect of leptin in proliferating and differentiated HC11 mouse mammary cells. Regul Pept. (2003) 113 (1-3): 101-7. Baratta M., et al.
Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin_Levels in Rodents. Mediators Inflamm. (2015) 2015: 620919. Quiñones M., et al.

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