Mouse leptin ELISA kit is designed for measuring quantitative concentrations of leptin using mouse samples of plasma, serum and cell culture supernatant. This assay has a minimum sensitivity detection limit of 0.86 pg/ml.
Leptin is a hormone which is important in regulating food intake, appetite and body weight. It is released from fat cells that are located in the adipose tissue and it can act as a specific receptor in the hypothalamus in order to inhibit appetite through a combination of stimulatory and counteractive mechanisms. A number of studies have also indicated that the absence of leptin within the body or leptin resistance can result in uncontrolled weight gain and feeding. Leptin in unable to affect food intake from one meal to another but instead acts in order to alter food intake and control energy expenditure over the long term. There is also a direct correlation between the levels of leptin circulating around the body and the amount of body fat, i.e. the greater the body fat levels then the more leptin that is present within the blood. A rare genetic condition where the body in unable to produce leptin is called congenital leptin deficiency, this rare condition makes the body think it has zero fat and leads to severe childhood obesity due to uncontrolled food intake. Leptin injections are available in order to treat this condition and this can result in dramatic weight loss.
MOUSE LEPTIN ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Pre-Coated Plate: Anti-mouse leptin rabbit IgG.
- Labelled Antibody (30x Conc.).
- Standard: Recombinant mouse leptin.
- EIA Buffer.
- Solution For Labelled Antibody.
- Chromogen (TMB).
- Stop Solution.
- Wash Buffer (40x Conc.).
The minimum sensitivity detection limit of leptin using current mouse leptin ELISA kit was approximately 0.86 pg/ml. The dynamic range for this assay is 12.5 – 800 pg/ml.
– Mouse Leptin: ELISA
– Titer Assay: 83.7 – 110.7%
– Added Recovery Assay: 84.1 – 102.0%
– Intra – Assay: 4.8 – 5.6%
– Inter – Assay: 4.8 – 5.6%
– Specificity: Mouse Leptin (100.0%), Rat Leptin (43.6%), Human Leptin (0.3%)
– Sensitivity: 0.86 pg/mL
- Leptin-independent programming of adult body weight and adiposity in mice Endocrinology. (2011) 152 (2): 476-82. Cottrell E.C., et al.
- Determination of the half-life of circulating leptin in the mouse. Int J Obes (Lond). (2017) 41 (3): 355-359. Burnett L.C., et al.
- Leptin reduces pathology and improves memory in a transgenic mouse model of Alzheimer’s disease. J Alzheimers Dis. (2010) 19 (4): 1155-67. Greco S.J., et al.
- Endothelial cell leptin receptor mutant mice have hyperleptinemia and reduced tissue uptake. J Cell Physiol. (2013) 228 (7): 1610-6. Hsuchou H., et al.
- Dimorphic effects of leptin on the circadian and hypocretinergic systems of mice. J Neuroendocrinol. (2011) 23 (1): 28-38. Mendoza J., et al.
- Full Name: Leptin ELISA Kit (Mouse)
- Reactivity: Mouse
- Sample Type: Serum, Plasma (EDTA), Cell Culture Supernant
- Sensitivity: 0.86 pg/ml