Mouse Leptin ELISA Kit

Full Name: Mouse Leptin ELISA Kit
Reactivity: Mouse
Sample Type: Serum, Plasma (EDTA), Cell Culture Supernant
Sensitivity: 0.86 pg/ml

BACKGROUND

Leptin is crucial for controlling appetite, food intake and body weight. It can operate as a particular receptor in the hypothalamus to reduce appetite through a mix of stimulatory and counteractive methods. It is secreted from fat cells found in adipose tissue. A number of studies have also indicated that the absence within the body or leptin-resistance can result in uncontrolled weight gain and feeding. behaviour. When this happens, weight gain may occur despite an apparently normal caloric intake. Leptin resistance may contribute to the onset of obesity, according to some data.

Leptin in unable to affect food intake from one meal to another but instead acts in order to alter food intake and control energy expenditure over the long term. The quantity of body fat and the levels present in the blood have a direct relationship i.e. the greater the body fat levels then the more leptin that is present within the blood. A rare genetic condition where the body in unable to produce-leptin is called congenital leptin deficiency, this rare condition makes the body think it has zero fat and leads to severe childhood obesity due to uncontrolled food intake. Leptin-injections are available in order to treat this condition and this can result in dramatic weight loss.

INTENDED USE

Mouse leptin ELISA kit is designed for measuring quantitative concentrations of leptin using mouse samples of plasma, serum and cell culture supernatant. This assay has a minimum sensitivity detection limit of 0.86 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Pre-Coated Plate: Anti-mouse leptin rabbit IgG.
Labelled Antibody (30x Conc.).
Standard.
EIA Buffer.
Solution For Labelled Antibody.
Chromogen (TMB).
Stop Solution.
Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of leptin using current mouse leptin ELISA kit was approximately 0.86 pg/ml. The dynamic range for this assay is 12.5 – 800 pg/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 83.7 – 110.7%
– Added Recovery Assay: 84.1 – 102.0%
– Intra – Assay: 4.8 – 5.6%
– Inter – Assay: 4.8 – 5.6%
– Specificity: Mouse Leptin (100.0%), Rat Leptin (43.6%), Human Leptin (0.3%)
– Sensitivity: 0.86 pg/mL

REFERENCES

Leptin-independent programming of adult body weight and adiposity in mice Endocrinology. (2011) 152 (2): 476-82. Cottrell E.C., et al.
Determination of the half-life of circulating_leptin in the mouse. Int J Obes (Lond). (2017) 41 (3): 355-359. Burnett L.C., et al.
Leptin reduces pathology and improves memory in a transgenic mouse model of Alzheimer’s disease. J Alzheimers Dis. (2010) 19 (4): 1155-67. Greco S.J., et al.
Endothelial cell leptin-receptor mutant mice have hyperleptinemia and reduced tissue uptake. J Cell Physiol. (2013) 228 (7): 1610-6. Hsuchou H., et al.

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