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Lactoferrin Ab ELISA Kit

INTENDED USE

Human lactoferrin antibody ELISA kit is intended for analysing in-vitro quantitative amounts of IgG auto-antibodies to lactoferrin (lactoferrin Ab, LF, lactotransferrin, LTF, lactoferrin IgG) in plasma or serum. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

BACKGROUND

Lactoferrin (LF) which can also be called lactotransferrin (LTF) is an iron binding protein which belongs to the transferrin family that is involved in the innate immune system. Apart from its main biological role of binding and transport of iron, it also possesses antiviral, anti-cancer, antibacterial, catalytic, anti-allergic and antiparasitic properties and functions. Lactoferrin mainly resides in specific granules of the polymorph nuclear neutrophil leukocytes (PMN) and can be present during secretions for example in tears, saliva or milk. Auto-antibodies against lactoferrin belong to the pANCA group and these are found to be at elevated levels in patients with ulcerative colitis, primary sclerosing cholangitis and rheumatoid vasculitis. During many active inflammatory disease, the higher serum concentrations of lactoferrin can be measured.

LACTOFERRIN ANTIBODY ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Divisible Microplate: Consisting of 12 modules of 8 wells, highly purified lactoferrin Ab is bound to microwells.
  • Calibrators A-F: Concentration 0, 6.3, 12.5, 25, 50, 100 U/ml.
  • Control Positive and Control Negative: Containing human lactoferrin antibody in a serum/buffer matrix.
  • Sample Buffer P (5x).
  • Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
  • TMB Substrate.
  • Stop Solution: Contains acid.
  • Wash Buffer (50x).

SENSITIVITY

The minimum detection sensitivity level of lactoferrin IgG autoantibodies (LTF, lactoferrin Ab, lactotransferrin, LF, lactoferrin IgG) using current lactoferrin antibody ELISA kit was 0.5 U/ml. The dynamic range for this assay is 6.3 – 100.0 U/ml.

ASSAY CHARACTERISTICS

– Lactoferrin Antibody: ELISA
– Results Interpretation: Serum samples from healthy blood donors displayed normal < 10 U/ml, elevated > 10 U/ml.
– Sensitivity: 0.5 U/ml
– Specificity: Highly specific only for autoantibodies directed against Lactoferrin Ab. No cross reactivities to the other ANCA antigens have been observed.
– Parallelism: Displayed linearity over the full measuring range.
– Interfering Substances: None

REFERENCES

  1. Immunomodulatory effects of lactoferrin. Acta Pharmacol Sin. (2014) 35 (5): 557-66. Review. Siqueiros-Cendón T., et al.
  2. Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine. Molecules. (2016) 21 (6). pii: E752. Review. Bruni N., et al.
  3. Lactoferrin: structure, function and applications. Int J Antimicrob Agents. (2009) 33 (4): 301.e1-8. Review. González-Chávez S.A., et al.
  4. LF immunomodulatory strategies: mastering bacterial endotoxin. Biochem Cell Biol. (2012) 90 (3): 269-78. Review. Latorre D., et al.
  5. Lactoferrin, a key molecule in immune and inflammatory processes. Biochem Cell Biol. (2012) 90 (3): 252-68. Review. Legrand D.
  6. Lactoferricin: a lactoferrin-derived peptide with antimicrobial, antiviral, antitumor and immunological properties. Cell Mol Life Sci. (2005) 62 (22): 2588-98. Review. Gifford J.L., et al.

ADDITIONAL INFORMATION

  • Full Name: Lactoferrin Ab ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.5 U/ml

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