GET A QUOTE

L-Selectin (CD62L) ELISA Kit

  • Created on the 3 May, 2017.

BACKGROUND

L-selectin (also referred to as CD62L) is found predominately on lymphocytes and has a main function of a homing receptor, aiding lymphocytes to enter into secondary lymphoid tissues through the high endothelial venules. The molecule itself is made up of many domains which include one homologous to EGF, one to lectins and two consensus repeat units which are present in C3/C4 binding proteins. The receptor is mostly located on the cell surface of T cells and also can be found in human embryo trophoblasts before the implantation stage into the uterus. There is also high expression of L-selectin present in human bone marrow progenitor cells and this has been used as a good indicator of cells that are committed to undergoing lymphoid differentiation.

INTENDED USE

Human L-selectin ELISA kit is intended for measuring in vitro quantitative amounts of l-selectin (CD62L) in human serum, cell lysates, cell culture supernatant, other biological fluids and plasma. This assay has a minimum sensitivity limit of 10.18 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of CD62L using this human L-selectin ELISA kit was 10.18 ng/ml. The dynamic assay range for this kit is 20.0 – 6,000 ng/ml.

REFERENCES

  1. Ligands for L-selectin: homing, inflammation, and beyond. Annu Rev Immunol. (2004) 22: 129-56. Review. Rosen S.D.
  2. Cell adhesion molecules in human embryo implantation. Sheng Li Xue Bao. (2012) 64 (3): 247-58. Review. Fukuda M.N. and Sugihara K.
  3. Selectins: initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovasc Res. (2015) 107 (3): 331-9. Review. McEver R.P.
  4. L-selectin–a dynamic regulator of leukocyte migration. Eur J Cell Biol. (2012) 91 (4): 257-64. Review. Wedepohl S., et al.
  5. CD62L (L-selectin). J Biol Regul Homeost Agents. (2002) 16 (2): 144-6. Review. Nicholson I.C.
  6. High-endothelial-venule ligands for L-selectin: identification and functions. Biochem Soc Trans. (1997) 25 (2): 428-33. Rosen S.D., et al.

ADDITIONAL INFORMATION

  • Full Name: L-Selectin (CD62L) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Cell Lysates, Other Biological Fluids, Serum, Cell Culture Supernatant
  • Sensitivity: 10.18 ng/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford