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JO-1 Ab ELISA Kit

  • Created on the 12 June, 2017.

BACKGROUND

Jo 1 (also known as histidyl tRNA synthetase) belongs to the family of amino acyl-tRNA synthetase enzymes which can be found in all nucleated cells. Jo 1 antibodies are important markers for the disease polymyositis (usually in myositis patients who also have interstitial lung disease). They are able to bind to conformational epitopes of the enzyme protein and result in inhibiting its catalytic activity. Jo1 is also referred to as an extractable nuclear antigen and can be detected using and ENA screen. It is found in over 25% of cases of polymyositis and also displays a good correlation with pulmonary fibrosis being present. Between 20–40% of polymyositis are found to be positive for Jo-1 antibodies, with majority also displaying interstitial lung disease, HLA-DR3 and HLA-DRw52 markers and these are often   collectively known as Jo-1 syndrome.

INTENDED USE

Human JO-1 ELISA kit is untended for detecting in-vitro quantitative concentrations of IgG autoantibodies to Jo 1 in human plasma and serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human Jo 1 IgG autoantibodies using this human Jo-1 ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 12.5 – 200.0 U/ml.

REFERENCES

  1. The role of Jo-1 in the immunopathogenesis of polymyositis: current hypotheses. Curr Rheumatol Rep. (2003) 5 (6): 425-30. Review. Ascherman D.P.
  2. Characterization and peripheral blood biomarker assessment of anti-Jo-1 antibody-positive interstitial lung disease. Arthritis Rheum. (2009) 60 (7): 2183-92. Richards T.J., et al.
  3. [Anti-Jo-1 autoantibodies (anti-histidyl tRNA synthetase autoantibodies)]. Nihon Rinsho. (1999) 57 Suppl: 431-4. Review. Japanese. Hirakata M. and Mimori T.
  4. Myopathy with anti-Jo-1 antibodies: pathology in perimysium and neighbouring muscle fibres. J Neurol Neurosurg Psychiatry. (2000) 68 (4): 472-8. Mozaffar T. and Pestronk A.
  5. Role of Jo-1 in the Immunopathogenesis of the Anti-synthetase Syndrome. Curr Rheumatol Rep. (2015) 17 (9): 56. Review. Ascherman D.P.

ADDITIONAL INFORMATION

  • Full Name: Jo-1 Ab ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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