IL-9 ELISA Kit (Interleukin-9)

INTENDED USE

Human IL-9 ELISA kit is designed for detecting in vitro quantitative concentrations of interleukin-9 (IL-9) present in human tissue homogenates, serum, plasma, cell culture supernatant and many other biological fluids. This assay has a minimum detection limit of 9.375 pg/ml.

BACKGROUND

Interleukin-9 (IL-9) is a pro-inflammatory cytokine, it was originally identified as a 40KD glycoprotein with T-cell growth factor activity in mice. It was later discovered in humans on the basis of its high level of homogeneity with the mouse gene. It has be strongly associated with human malignant lymphoma and also demonstrated to be a stimulator for hematopoiesis. Its expression is implicated with autoimmune and many allergic diseases. It is well studied as a therapeutic target for asthma due to it being implication with the disease.  It is be produced by Th2 cells which are under the stimulation of TGF-b and IL-4. This lineage has been shown to play vital role in the differentiation of Th17 cells and Treg function.

There has been a renewed attention on IL-9 in recent years due to a unique type of innate lymphoid cells which have been discovered in mice where the main cell types that are expressing IL-9 secreting and in vivo cells present within the skin appear to mediate the inhibition of melanoma growth. These same secreting cells have now also been identified in human and are being evaluated as a new tumour treatment strategy.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human IL-9 antibody.
• Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled-Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-9 (IL-9) using this IL-9 ELISA kit was approximately 9.375 pg/ml. The dynamic range for this assay is 15.625 – 1000 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-9, no cross reactivity or interference between IL-9 and analogues was detected.
– Recovery: Serum (88 – 101%), EDTA Plasma (85 – 104%), Heparin Plasma (87 – 104%).
– Linearity: Serum (91 – 103%), EDTA Plasma (82 – 94%), Heparin Plasma (82 – 89%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Cellular sources and immune functions of interleukin-9. Nat Rev Immunol. (2010) 10 (10): 683-7. Review. Noelle R.J. and Nowak E.C.
2.  A brief history of IL-9. J Immunol. (2011) 186 (6): 3283-8. Review. Goswami R. and Kaplan M.H.
3. Th9 cells: differentiation and disease. Immunol Rev. (2013) 252 (1): 104-15. Review. Kaplan M.H.
4. Role of IL-9 in the pathophysiology of allergic diseases. J Allergy Clin Immunol. (2001) 107 (4): 575-82. Review. Soussi-Gounni A., et al.
5. Interleukin 9 and its receptor: an overview of structure and function. Int Rev Immunol. (1998) 16 (3-4): 345-64. Review. Demoulin J.B. and Renauld J.C.

ADDITIONAL INFORMATION

• Full Name: IL-9 ELISA Kit (Interleukin-9)
• Reactivity: Human
• Sample Type: Serum, Plasma, Other Biological Fluids, Tissue Homogenates
• Sensitivity: 9.375 pg/ml

OTHER RELATED ELISA KITS

• TGF-Alpha ELISA Kit
• IL-4 ELISA Kit
• IL-2 ELISA Kit