IL-8 ELISA Kit (Interleukin-8)

INTENDED USE

Human IL-8 ELISA kit is intended for measuring in vitro quantitative levels of interleukin-8 (IL-8) present in human serum, tissue homogenates, serum and other biological fluids. This assay has a minimum detection limit of 18.75 pg/ml.

BACKGROUND

Macrophages and other cell types including; epithelial cells, airway smooth muscle cells, and endothelial cells all generate interleukin 8 (IL-8). It is kept by endothelial cells in their Weibel-Palade bodies, which serve as storage vesicles. It is originally synthesised as a 99-amino-acid precursor peptide, which is then cleaved to produce a number of active isoforms. In culture, macrophages primarily produce a 72 amino acid peptide.

IL-8 performs two primary functions. First, it causes chemotaxis in target cells, which causes them to move towards the infection site. As soon as these cells reach the area, it also triggers phagocytosis. Second, it is a potent angiogenesis promoter. It causes a variety of physiological processes, including increased intracellular Ca2+, exocytosis (e.g., the release of histamine), and the respiratory burst, which are all necessary for cells to move and ingest substances, when it is present in target cells.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human IL-8 antibody.
• Standards: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-8 (IL-8) using this IL-8 ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-8, no cross reactivity or interference between IL-8 and analogues was detected.
– Recovery: Serum (85 – 105%), EDTA Plasma (86 – 102%), Heparin Plasma (92 – 100%).
– Linearity: Serum (88 – 101%), EDTA Plasma (83 – 98%), Heparin Plasma (85 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Interleukin 8 and acute lung injury. Arch Pathol Lab Med. (2014) 138 (2): 266-9. Review. Allen T.C. and Kurdowska A.
2. Roles of IL-8 in ocular inflammations: a review. Ocul Immunol Inflamm. (2011) 19 (6): 401-12. Review. Ghasemi H., et al.
3. Mitogenic effects of interleukin-8/CXCL8 on cancer cells. Future Oncol. (2005) 1 (5): 699-704. Review. Zhu Y.M. and Woll P.J.
4. Neutrophil receptors for interleukin-8 and related CXC chemokines. Semin Hematol. (1997) 34 (4): 311-8. Review. Murphy P.M.

ADDITIONAL INFORMATION

• Full Name: IL-8 ELISA Kit (Interleukin-8)
• Reactivity: Human
• Sample Type: Serum, Plasma (Heparin, EDTA, Citrate), Other Biological Fluids, Tissue Homogenates
• Sensitivity: 18.75 pg/ml

OTHER RELATED ELISA KITS

• IL-1 Beta ELISA Kit
• IL-16 ELISA Kit
• IL-3 ELISA Kit