Interleukin-8 (IL-8) ELISA Kit


Interleukin-8 ELISA kit is intended for measuring in vitro quantitative levels of interleukin-8 (IL-8) present in human serum, cell culture supernatant and plasma (EDTA, citrate, heparin). This assay has a minimum detection limit of < 1.0 pg/ml.


Interleukin-8 (IL-8) which can also be known as T lymphocyte chemotactic factor (TCF), monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil attractant/activating protein (NAP-1), leukocyte adhesion inhibitor (LAI) and monocyte-derived neutrophil-activating peptide (MONAP). IL-8 belongs to a chemokine superfamily that has the ability to selectively activate and chemo-attract specific leukocyte subpopulations, all of these are cytokines which contain four conserved cysteines and can be divided into two distinguishable subfamilies (C-X-C proteins or CC-protein). IL-8 is a member of the C-X-C subfamily alongside platelet basic protein (PBP), neutrophil-activating peptide-2 (NAP-2), platelet factor 4 (PF4), g-interferon-inducible protein (g-IP-10), connective-tissue-activating peptide III (CTAPIII), MGSA/CRO gene products (GRO-a, GRO-b, GRO-g) and b-thromboglobulin, ENA-78.

The following interleukin-8 ELISA kit can be used to detect many different types of diagnostic applications. IL-8 is found to be produced by a number of cells in response to inflammatory stimuli examples include TNF-a, IL-1b, mitogen, viruses, lectins, phorbol esters (PMA) and crystals. There are many cell types which are producing IL-8 in response to these stimuli such as fibroblasts, endothelial cells, neutrophils, keratinocytes, chondrocytes, mesothelial cells, glioblatoma cells, T lymphocytes, monocytes and macrophages. There are also various non-infectious human diseases which are linked with neutrophilia and neutrophil infiltration into organs. Some examples of these human diseases include immune vasculitis, psoriasis, rheumatoid arthritis, glomerulonephritis, gouty arthritis, inflammatory bowel disease, acute meningococcal infections, cystic fibrosis and alcoholic hepatitis. This interleukin-8 ELISA kit offers a sensitivity level of 1.0 pg/ml and can be used to measure human samples of plasma, cell culture supernatant and serum.


The minimum detection sensitivity level of IL-8 (interleukin-8) using current human interleukin-8 ELISA kit was approximately 1.0 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.


  1. Interleukin 8 and acute lung injury. Arch Pathol Lab Med. (2014) 138 (2): 266-9. Review. Allen T.C. and Kurdowska A.
  2. Roles of IL-8 in ocular inflammations: a review. Ocul Immunol Inflamm. (2011) 19 (6): 401-12. Review. Ghasemi H., et al.
  3. Interleukin-8 and human cancer biology. Cytokine Growth Factor Rev. (2001) 12 (4): 375-91. Review. Xie K.
  4. Mitogenic effects of interleukin-8/CXCL8 on cancer cells. Future Oncol. (2005) 1 (5): 699-704. Review. Zhu Y.M. and Woll P.J.
  5. Neutrophil receptors for interleukin-8 and related CXC chemokines. Semin Hematol. (1997) 34 (4): 311-8. Review. Murphy P.M.


  • Full Name: Interleukin-8 (IL-8) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (Heparin, EDTA, Citrate), Cell Culture Supernatant
  • Sensitivity: < 1.0 pg/ml



Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford