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Interleukin-8 (IL-8) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-8 (IL-8) which can also be known as T lymphocyte chemotactic factor (TCF), monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil attractant/activating protein (NAP-1), leukocyte adhesion inhibitor (LAI) and monocyte-derived neutrophil-activating peptide (MONAP). IL-8 belongs to a chemokine superfamily that has the ability to selectively activate and chemo-attract specific leukocyte subpopulations, all of these are cytokines which contain four conserved cysteines and can be divided into two distinguishable subfamilies (C-X-C proteins or CC-protein). IL-8 is a member of the C-X-C subfamily alongside platelet basic protein (PBP), neutrophil-activating peptide-2 (NAP-2), platelet factor 4 (PF4), g-interferon-inducible protein (g-IP-10), connective-tissue-activating peptide III (CTAPIII), MGSA/CRO gene products (GRO-a, GRO-b, GRO-g) and b-thromboglobulin, ENA-78.

Interleukin-8 is found to be produced by a number of cells in response to inflammatory stimuli examples include TNF-a, IL-1b, mitogen, viruses, lectins, phorbol esters (PMA) and crystals. There are many cell types which are producing IL-8 in response to these stimuli such as fibroblasts, endothelial cells, neutrophils, keratinocytes, chondrocytes, mesothelial cells, glioblatoma cells, T lymphocytes, monocytes and macrophages. There are also various non-infectious human diseases which are linked with neutrophilia and neutrophil infiltration into organs. Some examples of these human diseases include immune vasculitis, psoriasis, rheumatoid arthritis, glomerulonephritis, gouty arthritis, inflammatory bowel disease, acute meningococcal infections, cystic fibrosis and alcoholic hepatitis.

INTENDED USE

Interleukin-8 ELISA kit is intended for measuring in vitro quantitative levels of interleukin-8 (IL-8) present in human serum, cell culture supernatant and plasma (EDTA, citrate, heparin). This assay has a minimum detection limit of < 1.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-8 (IL-8) using this human interleukin-8 ELISA kit was approximately 1.0 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000 pg/ml.

REFERENCES

  1. Interleukin 8 and acute lung injury. Arch Pathol Lab Med. (2014) 138 (2): 266-9. Review. Allen T.C. and Kurdowska A.
  2. Roles of IL-8 in ocular inflammations: a review. Ocul Immunol Inflamm. (2011) 19 (6): 401-12. Review. Ghasemi H., et al.
  3. Interleukin-8 and human cancer biology. Cytokine Growth Factor Rev. (2001) 12 (4): 375-91. Review. Xie K.
  4. Mitogenic effects of interleukin-8/CXCL8 on cancer cells. Future Oncol. (2005) 1 (5): 699-704. Review. Zhu Y.M. and Woll P.J.
  5. Neutrophil receptors for interleukin-8 and related CXC chemokines. Semin Hematol. (1997) 34 (4): 311-8. Review. Murphy P.M.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-8 (IL-8) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (Heparin, EDTA, Citrate), Cell Culture Supernatant
  • Sensitivity: < 1.0 pg/ml

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