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Interleukin-7 (IL-7) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-7 (IL-7) is a hematopoietic growth factor that is secreted by stromal cells in the thymus or red marrow, it is essentially a 17.4 kDa protein containing six exons. IL-7 is produced by endothelial cells, dendritic cells, keratinocytes, hepatocytes and neurons. It has the ability to affect survival and proliferation of mature T cells, enhances proliferation and survival of T cell precursors (human and mice) and B cell precursors (mice only), potent enhancer of T cell function and IFN-γ production. Interleukin-7 is a critical cytokine for both normal T and B lymphopoiesis, a mobilizer of pluripotent stem cells and myeloid progenitors. Possessing vital roles in enhancing T cell function and also able to induces cytokine expression in monocytes.  

Preclinical studies have indicated that IL-7 may be capable of accelerating murine lymphocyte regeneration following chemotherapy or bone marrow transplantation treatment, inducing anti-tumor effects (mice only) and useful in expanding anti-HIV-specific human T cells. The levels of IL-7 are found to be elevated in untreated patients with advanced HIV disease or in those who do not respond well to highly active antiretroviral therapy (HAART), whereas IL-7 levels are undetectable in HIV-infected patients who respond well to HAART treatment. Therefore, it is important to continue to evaluate IL-7 as a potential therapy for HIV/AIDS, leukopenia, stem cell transplantation and bone marrow cancer in both pre-clinical and clinical research settings.

INTENDED USE

Interleukin-7 ELISA kit is a procedure of detecting in vitro quantitative amounts of interleukin-7 (IL-7) present in human serum, cell culture supernatant and EDTA-plasma. This assay has a minimum detection limit of < 1.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-7 (IL-7) using this human interleukin-7 ELISA kit was approximately 1.0 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000 pg/ml.

REFERENCES

  1. Interleukin 7 and thymic stromal lymphopoietin: from immunity to leukemia. Cell Mol Life Sci. (2014) 71 (3): 365-78. Review. Tal N., et al.
  2. Interleukin-7 (IL-7): immune function, involvement in the pathogenesis of HIV infection and therapeutic potential. Eur Cytokine Netw. (2004) 15 (4): 279-89. Review. Beq S., et al.
  3. Interleukin-7 and immune reconstitution in cancer patients: a new paradigm for dramatically increasing overall survival. Target Oncol. (2012) 7 (1): 55-68. Review. Morre M. and Beq S.
  4. Mechanism of Action of IL-7 and Its Potential Applications and Limitations in Cancer Immunotherapy. Int J Mol Sci. (2015) 16 (5): 10267-80. Review. Gao J., et al.
  5. Interleukin 7 receptor alpha as a potential therapeutic target in transplantation. Arch Immunol Ther Exp (Warsz). (2009) 57 (4): 253-61. Review. Racapé M., et al.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-7 (IL-7) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Cell Culture Supernatant, EDTA-Plasma
  • Sensitivity: < 1.0 pg/ml

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