GET A QUOTE

Interleukin-5 (IL-5) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-5 (IL-5) is also commonly referred to as eosinophil differentiation factor (EDF), it is a 135 amino acid polypeptide with a molecular mass of 12.5 kDa. Monomeric IL-5 has no activity and needs to form a homodimer in order for it to become active and function. One IL-5 homodimer has the ability to engage in binding to one IL-5 receptor and it has been implicated that the dimer undergoes a conformational change upon binding a receptor molecule and this change allows it to bind to a second receptor. The amino acid sequence of mature human and mouse are approx. 75% identical, however, the human IL-5 is marginally active on mouse cells whereas mouse IL-5 is highly active on human cells.

Interleukin 5 along with the chemokine eotaxin play a vital role in the coordination of the eosinophil-based inflammatory response. Even though there are many cells that contribute to inflammation, eosinophils are noted in particular because of their contribution to late phase allergic-type disorders. When they are activated within tissues, eosinophils are able to release highly basic preformed mediators (e.g. major basic protein and eosinophil peroxidase). These substances are highly toxic to parasites and can be quite damaging to the surrounding tissue functionality such as superoxide-mediated tissue destruction, vascular permeability and smooth muscle constriction. IL-5 appears to carry out a number of functions on eosinophils which can include down modulation of Mac-1, growth and differentiation, induction of granule release, upregulation of receptors for IgA or IgG and the stimulation of lipid mediator (PAF and leukotriene C4) secretion. Finally, IL-5 has been considered as a candidate gene in the pathogenesis of asthma, since it is the main cytokine controlling eosinophil activity, eosinophils have been identified as being pivotal in the development of airway inflammation.

INTENDED USE

Interleukin-5 ELISA kit can be used for in vitro quantitative analysis of interleukin-5 (IL-5) levels in human cell culture supernatant, serum, plasma (EDTA, heparin) and urine samples. This assay has a minimum detection limit of < 2.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-5 (IL-5) using this human interleukin-5 ELISA kit was approximately 2.0 pg/ml. The dynamic assay range for this kit is 7.8 – 500 pg/ml.

REFERENCES

  1. Inhibition of interleukin-5 for the treatment of eosinophilic diseases. Discov Med. (2012) 13 (71): 305-12. Review. Corren J.
  2. Anti-interleukin-5 therapy in severe asthma. Eur Respir Rev. (2013) 22 (129): 251-7. Review. Garcia G., et al.
  3. Analysis of three human interleukin 5 structures suggests a possible receptor binding mechanism. FEBS Lett. (1998) 424 (3): 121-6. Review. Verschelde J.L., et al.
  4. Cytokine recognition by human interleukin 5 receptor. Vitam Horm. (2005) 71: 321-44. Review. Ishino T., et al.
  5. Anti-interleukin-5 antibody therapy in eosinophilic diseases. Pathobiology. (2005) 72 (6): 287-92. Review. Simon D., et al.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-5 (IL-5) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Cell Culture Supernatant, Plasma (Heparin, EDTA), Urine
  • Sensitivity: < 2.0 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford