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Interleukin-4 (IL-4) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-4 (IL-4) was first characterised as B cell stimulatory factor (BSF-1) and B-cell differentiation factor (BCDFg). It has been subsequently revealed that IL-4 is a pleiotropic cytokine with many immune response modulating functions on a large number of cell types which include fibroblasts, osteoblasts, endothelial cells, hepatocytes, keratinocytes, astrocytes, mast cells, macrophages and T cells. It is essentially an immunomodulatory cytokine that has the ability to inhibit the growth of tumour cells. Interleukin-4 is known to be produced by mast cells, fetal thymocytes, CD8+ T cells, basophils, CD4+ TH0 and TH2 cells.

The human cDNA is made up of a single open reading frame which encodes a protein of 153 amino acids and this also includes a putative signal peptide. The human IL-4 gene is located on chromosome 5 and it occurs as a single copy in the haploid genome. There are structural and functional similarities between human and mouse IL-4 genes and their proteins, both human and mouse proteins have multiple potential glycosylation sites and six cysteines which are linked by intro-molecular disulfide bridges. There are evidence that IL-4 can act as an autocrine growth factor in pancreatic cancer cells, this has been found to give rise to the possibility that cancer-derived IL-4 being able to suppress the cancer-directed immune-surveillance in vivo, in addition to its growth-promoting effects. This process can result in the facilitation of both pancreatic tumor growth and metastasis. The standard used in this ELISA assay is recombinant human IL-4 that is composed of 130 amino acids with a molecular mass of 14KDa.

INTENDED USE

Interleukin-4 ELISA kit is designed for measuring in vitro quantitative concentrations of interleukin-4 (IL-4) present in human cell culture supernatant, plasma (EDTA, heparin, citrate) and serum samples. This assay has a minimum detection limit of < 1.5 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-4 (IL-4) using this human interleukin-4 ELISA kit was approximately 1.5 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000 pg/ml.

REFERENCES

  1. Control of IL-4 expression in T helper 1 and 2 cells. Immunology. (2008) 124 (4): 437-44. Review. Gilmour J. and Lavender P.
  2. IL-4 in the brain: a cytokine to remember. J Immunol. (2012) 189 (9): 4213-9. Review. Gadani S.P., et al.
  3. Spatial regulation of IL-4 signalling in vivo. Cytokine. (2015) 75 (1): 51-6. Review. Redpath S.A., et al.
  4. Anti-interleukin-4 therapy. Immunol Allergy Clin North Am. (2004) 24 (4): 599-614. Review. Steinke J.W.
  5. History of interleukin-4. Cytokine. (2015) 75 (1): 3-7. Review. Paul W.E.
  6. Gene therapy and biologic therapy with interleukin-4. Curr Gene Ther. (2002) 2 (4): 437-50. Review. Okada H. and Kuwashima N.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-4 (IL-4) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture Supernatant, Plasma (Heparin, EDTA, Citrate), Serum
  • Sensitivity: < 1.5 pg/ml

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