Interleukin-3 ELISA kit is a procedure for analysing in vitro quantitative levels of interleukin-3 (IL-3) in human plasma (heparin, EDTA, citrate), serum and cell culture supernatant samples. This assay has a minimum detection limit of < 0.1 pg/ml.
Interleukin-3 (IL-3) is essentially a hematopoietic colony-stimulating factor (CSF) which has the ability to support the proliferation of a large number of hematopoietic cell types. It is a protein that is 140 amino acids in length which is chemically synthesised using an automated peptide synthesiser and this displays biological activities that can be attributed to native IL-3. The gene IL-3 in known to encode interleukin 3, where the human gene encoding IL-3 is found to be tandemly arrayed on the long arm of chromosome 5.
The mouse IL-3 gene is located on chromosome 11 and the cDNA sequence for codes for a polypeptide of 166 amino acids including a putative signal peptide. It is regarded as one of the colony stimulating factors that is vital in regulating haematopoiesis. The standard used in the following interleukin-3 ELISA kit is a recombinant human IL-3 that is composed of 133 amino acids having a total molecular mass of 15KDa.
The minimum detection sensitivity level of IL-3 (interleukin-3) using current human interleukin-3 ELISA kit was approximately 0.1 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.
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- Full Name: Interleukin-3 (IL-3) ELISA Kit
- Reactivity: Human
- Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA, Citrate, Heparin)
- Sensitivity: < 0.1 pg/ml