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Interleukin-18 (IL-18) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-18 (IL-18) which is also referred to as interferon-gamma-inducing factor (IGIF) and is a non-glycosylated 24 kDa polypeptide which is known to lack any classical signal sequence. IL-18 is a cytokine which is member of the IL-1 superfamily and is primarily produced by macrophages and many other cells. Interleukin-18 is synthesized as a bio-inactive pro-peptide which then undergoes proteolytic cleavage by either caspase enzymes or interleukin-1 beta converting enzyme (ICE) in order to generate a mature 18 kDa bioactive molecule. IL-18 is a recently identified cytokine that has the ability to modulate both T helper type 1 (Th1) and Th2 responses.

In order for it to carry out its function it needs to bind to the interleukin-18 receptor and together with together with IL-12 it has the ability to induce cell-mediated immunity after an infection with microbial products such as lipopolysaccharide (LPS). Apart from its physiological role, IL-18 is found to induce many severe inflammatory reactions, this has led to a suggestion of possible roles in particular inflammatory disorders. It has been implicated as a mediator of Hashimoto’s thyroiditis and found to increase Alzheimer’s disease-associated amyloid-beta production in many human neuron cells. IL-18 is also up regulated by interferon-gamma and is a potent pro-inflammatory cytokine with potential atherogenic properties. The biologic effects of interleukin-18 therapy include activation of T cells, monocytes, NK cells, production of IFN-gamma as well as other cytokines in vivo. Il-18 is found to act as a co-stimulatory cytokine and its optimal use as a cancer immune-therapy is possible with the combination of other immuno-stimulatory cytokines, monoclonal antibodies and vaccines.

INTENDED USE

Interleukin-18 ELISA kit is a method for detecting in vitro quantitative concentrations of interleukin-18 (IL-18) in human serum, plasma (EDTA, heparin) and cell culture supernatant. This assay has a minimum detection limit of < 1.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-18 (IL-18) using this human interleukin-18 ELISA kit was approximately 1.0 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000 pg/ml.

REFERENCES

  1. Interleukin-18: recent advances. Curr Opin Hematol. (2004) 11 (6): 405-10. Review. Reddy P.
  2. IL-18: a new player in immunotherapy for age-related macular degeneration? Expert Rev Clin Immunol. (2014) 10 (10): 1273-5. Review. Campbell M., et al.
  3. Interleukin-18, more than a Th1 cytokine. Semin Immunol. (2013) 25 (6): 439-48. Review. Novick D., et al.
  4. IL-18 in inflammatory and autoimmune disease. Cell Mol Life Sci. (2013) 70 (24): 4795-808. Review. Sedimbi S.K., et al.
  5. The role of interleukin-18 in the metabolic syndrome. Cardiovasc Diabetol. (2010) 9: 11. Review. Trøseid M., et al.
  6. Immunotherapeutic applications of IL-18. Immunotherapy. (2012) 4 (12): 1883-94. Review. Tsutsui H. and Nakanishi K.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-18 (IL-18) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma (Heparin, EDTA), Cell Culture Supernatant, Serum
  • Sensitivity: < 1.0 pg/ml

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