IL-17 ELISA Kit (Interleukin-17)

INTENDED USE

Human IL-17 ELISA kit is designed for analysing in vitro quantitative levels of interleukin-17 (IL-17) in human plasma (EDTA, heparin), serum, biological fluids and tissue homogenates samples. This assay has a minimum detection limit of 3.75 pg/ml.

BACKGROUND

Interleukin-17 (IL-17 or IL-17A) is a secreted glycoprotein with a molecular mass of 35 kDa, it is a 155 amino acids protein that is joined by a disulphide-linked. The structure is made up of a signal peptide (approx. 23 amino acids) that is followed by a 123 amino acid chain region which is characteristic of the family. In order for it to carry out its function it needs to bind to a type I cell surface receptor called IL-17R (can be either IL17RA, IL17RB or IL17RC).

All members of the IL-17 family display a very similar protein structure, consisting of four highly conserved cysteine residues that are essential for their 3-dimensional shape. These cytokines are also known to be well conserved in mammals, with approx. 62–88% of amino acid conservation between the human and mouse homologs. It is most notably known for inducing and mediating pro-inflammatory responses, it has also been commonly associated with allergic responses.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human IL-17 antibody.
• Standards: Concentrations 0, 6.25, 12.5, 25, 50, 100, 200, 400 pg/ml.
• Sample/Standard Dilution Buffer.
• Assay Dilution.
• Biotinylated-labelled Antibody.
• HRP-Streptavidin (HRP-SA).
• Biotin System (BS).
• BS Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-17 (IL-17) using this IL-17 ELISA kit was approximately 3.75 pg/ml. The dynamic range for this assay is 6.25 – 400 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-17, no cross reactivity or interference between IL-17 and analogues was detected.
– Recovery: Serum (89 – 102%), EDTA Plasma (90 – 99%), Heparin Plasma (91 – 104%).
– Linearity: Serum (87 – 103%), EDTA Plasma (85 – 99%), Heparin Plasma (80 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. IL-17 in Chronic Inflammation: From Discovery to Targeting. Trends Mol Med. (2016) 22 (3): 230-41. Review. Beringer A., et al.
2. Update on the role of Interleukin 17 in rheumatologic autoimmune diseases. Cytokine. (2015) 75 (2): 207-15. Review. Konya C., et al.
3. Therapeutic Targeting of IL-17 and IL-23 Cytokines in Immune-Mediated Diseases. Annu Rev Med. (2016) 67: 337-53. Review. Fragoulis G.E., et al.
4. The IL-23 to IL-17-cascade inflammation-related cancers. Clin Exp Rheumatol. (2015) 33 (4 Suppl 92): S87-90. Review. Wang K. and Karin M.

ADDITIONAL INFORMATION

• Full Name: IL-17 ELISA Kit (Interleukin-17)
• Reactivity: Human
• Sample Type: Biological Fluids, Plasma (Heparin, EDTA), Tissue Homogenates, Serum
• Sensitivity: 3.75 pg/ml

OTHER RELATED ELISA KITS

• IL-15 ELISA Kit
• IL-10 ELISA Kit
• IL-7 ELISA Kit