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Interleukin-16 (IL-16) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-16 (IL-16) which was also previously known as lymphocyte chemoattractant factor (LCF), is essentially a lymphocyte chemo-attractant factor of T cell origin that as a selective activity for CD4+ T cells. IL-16 is a product of eosinophils, CD4+ cells, CD8+ cells, epithelial cells derived from asthmatics and mast cells. The expression of IL-16 in asthmatic epithelium is found to directly correlate with the number of infiltrating CD4+ T cells. In addition to its chemotactic activity, it is also a competence growth factor that is selective for CD4+ T cells that are dependent on an interaction with CD4 in order to induce their of functional activity.

Interleukin-16 cDNA is known to code for a novel 14-kDa protein, which needs to form a homo-tetrameric complex in order to induce biological activity. In culture supernatants IL-16 appears as a relative molecular mass (Mr) of approx. 56,000 biologically active, non-covalently linked tetramer, but is found to migrate in monomeric form in SDS PAGE. The eluted monomeric peptides are inactive but have the ability to re-aggregate to Mr 56,000 which allows them to regain biological activity. The protein that is expressed from the IL-16 cDNA is able to demonstrate all the functions and chemical features of the native protein.

INTENDED USE

Interleukin-16 ELISA kit is a procedure for detecting in vitro quantitative amounts of interleukin-16 (IL-16) in human plasma, serum, cell culture supernatant and other biological fluids. This assay has a minimum detection limit of < 20.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-16 (IL-16) using this human interleukin-16 ELISA kit was approximately 20.0 pg/ml. The dynamic assay range for this kit is 20.0 – 3,200 pg/ml.

REFERENCES

  1. Interleukin-16 network in inflammation and allergy. Allergy Asthma Proc. (2002) 23 (2): 103-8. Review. Conti P., et al.
  2. Not-so-sweet sixteen: the role of IL-16 in infectious and immune-mediated inflammatory diseases. J Interferon Cytokine Res. (2006) 26 (8): 511-20. Review. Glass W.G., et al.
  3. Signaling and functional properties of interleukin-16. Int Rev Immunol. (1998) 16 (5-6): 523-40. Review. Cruikshank W.W., et al.
  4. CD8+ T-cell-mediated non-cytolytic suppression of human immuno-deficiency viruses. Curr Drug Targets Infect Disord. (2003) 3 (2): 97-113. Review. Vella C. and Daniels R.S.
  5. lnterleukin-16: the ins and outs of regulating T-cell activation. Crit Rev Immunol. (2008) 28 (6): 467-83. Review. Cruikshank W. and Little F.
  6. Prospects for IL-16 in the treatment of AIDS. Expert Opin Biol Ther. (2001) 1 (3): 425-32. Review. Kornfeld H. and Cruikshank W.W.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-16 (IL-16) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum, Cell Culture Supernatant, Other Biological Fluids
  • Sensitivity: < 20.0 pg/ml

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