- Created on the 12 March, 2017.
Interleukin-16 (IL-16) which was also previously known as lymphocyte chemoattractant factor (LCF), is essentially a lymphocyte chemo-attractant factor of T cell origin that as a selective activity for CD4+ T cells. IL-16 is a product of eosinophils, CD4+ cells, CD8+ cells, epithelial cells derived from asthmatics and mast cells. The expression of IL-16 in asthmatic epithelium is found to directly correlate with the number of infiltrating CD4+ T cells. In addition to its chemotactic activity, it is also a competence growth factor that is selective for CD4+ T cells that are dependent on an interaction with CD4 in order to induce their of functional activity.
Interleukin-16 cDNA is known to code for a novel 14-kDa protein, which needs to form a homo-tetrameric complex in order to induce biological activity. In culture supernatants IL-16 appears as a relative molecular mass (Mr) of approx. 56,000 biologically active, non-covalently linked tetramer, but is found to migrate in monomeric form in SDS PAGE. The eluted monomeric peptides are inactive but have the ability to re-aggregate to Mr 56,000 which allows them to regain biological activity. The protein that is expressed from the IL-16 cDNA is able to demonstrate all the functions and chemical features of the native protein.
Interleukin-16 ELISA kit is a procedure for detecting in vitro quantitative amounts of interleukin-16 (IL-16) in human plasma, serum, cell culture supernatant and other biological fluids. This assay has a minimum detection limit of < 20.0 pg/ml.
The minimum detection sensitivity level of interleukin-16 (IL-16) using this human interleukin-16 ELISA kit was approximately 20.0 pg/ml. The dynamic assay range for this kit is 20.0 – 3,200 pg/ml.
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- Full Name: Interleukin-16 (IL-16) ELISA Kit
- Reactivity: Human
- Sample Type: Plasma, Serum, Cell Culture Supernatant, Other Biological Fluids
- Sensitivity: < 20.0 pg/ml