Interleukin-15 (IL-15) ELISA Kit

BACKGROUND

Interleukin-15 (IL-15), at maturity is able to reach 114 amino acid residues and is a modest 14-15 kDa glycoprotein that can be produced by dendritic and monocyte cells. IL-15 belongs to the four a helix bound cytokine family, other members include IL-2, IL-3, IL-5, IL-6, IL-7 and IL-9. At present the structure of IL-15 has not been determined, however, it has been predicted to be similar to IL-2 and other members of this family. The mRNA of IL-15 has been identified in monocytes, dendritic cells, epithelial cells, fibroblasts, placenta, kidneys, heart, skeletal muscle and lungs. Human IL-15 is known to display approximately 97% and 73% amino acid sequence identity with simian and mouse IL-15, respectively. Both it is found that human and simian IL-15 molecules are active with mouse cells.

Abnormal levels of IL-15 expression have been discovered in inflammatory bowel disease, rheumatoid arthritis and many diseases that have been associated with the retroviruses HIV and HTLV-I. The biological activities of IL-15 are very comparable to IL-2 such as IL-15 being able to induce the proliferation of activated CD4+, CD8+, CD4+CD8+, CD4-CD8- and dendritic epidermal T cells. The activation, development and survival of natural killer (NK) cells has been associated to IL-15. Interleukin-15 also affects the non-lymphoid tissues, such as brain, muscle, mast cells and microglia. In combination with recombinant CD40L, IL-15 can be a potent inducer of polyclonal IgA, IgG1 and IgM secretion, but it is unable to induce the production of IgE or IgG4. The use of anti-IL-15 therapy with the combination of classic immunosuppression therapy could be beneficial in the prevention or treatment of acute and especially chronic, allograft rejection.

This standard used in this ELISA assay is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

INTENDED USE

Interleukin-15 ELISA kit is intended for measuring in vitro quantitative concentrations of interleukin-15 (IL-15) in human plasma (EDTA, heparin), serum and cell culture supernatant. This assay has a minimum detection limit of < 3.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-15 (IL-15) using this human interleukin-15 ELISA kit was approximately 3.0 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000 pg/ml.

REFERENCES

1. IL15 and T-cell Stemness in T-cell-Based Cancer Immunotherapy. Cancer Res. (2015) 75 (24): 5187-93. Review. Pilipow K., et al.
2. Interleukin-15: new kid on the block for antitumor combination therapy. Cytokine Growth Factor Rev. (2015) 26 (1): 15-24. Van den Bergh J.M., et al.
3. IL-15 functions as a danger signal to regulate tissue-resident T cells and tissue destruction. Nat Rev Immunol. (2015) 15 (12): 771-83. Review. Jabri B. and Abadie V.
4. Interleukin-15 in the treatment of cancer. Expert Rev Clin Immunol. (2014) 10 (12): 1689-701. Review. Waldmann T.A.
5. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis. Mediators Inflamm. (2014) 2014: 561459. Review. Wojdasiewicz P., et al.
6. Therapeutic potential of IL-15 in rheumatoid arthritis. Hum Immunol. (2015) 76 (11): 812-8. Review. Yang X.K., et al.

ADDITIONAL INFORMATION

• Full Name: Interleukin-15 (IL-15) ELISA Kit
• Reactivity: Human
• Sample Type: Plasma (Heparin, EDTA), Serum, Cell Culture Supernatant
• Sensitivity: < 3.0 pg/ml

OTHER RELATED ELISA KITS

• Interleukin-2 (IL-2) ELISA Kit
• Interleukin-7 (IL-7) ELISA Kit
• Interleukin-9 (IL-9) ELISA Kit