- Created on the 12 March, 2017.
Interleukin-12 (IL-12) is a pleiotropic cytokine which also use to be called natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is primarily produced by stimulated macrophages. It is a 70-KDa cytokine that is composed of two disulfide-linked proteins (p35 and p40), IL-12p70 is a heterodimer of p35 and p40 subunits and it is a vital cytokine that is secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression studies have indicated that both strains of the IL-12 cytokine family can induce high levels of p40 (protein chain that is communal to both IL-12 p70 and IL-23) and p19 (a subunit of IL-23). Conversely it was found that only infection with ACT-18HS19 were able to consistently induced the transcription of IL-12 p35 (a subunit of IL-12 p70).
Interleukin-12 was originally discovered as factor being produced by human Epstein-Barr-Virus transformed B cell lines. It was also shown to be a pro-inflammatory cytokine that can be produced by B cells, phagocytic cells and many antigen-presenting cells that have the ability to modulate the adaptive immune responses by generating T-helper type 1 cells. IL-12 can exert a number of different biological effects on natural killer cells and human T cells. There is a strong suggestion of a potential usefulness in cancer therapy, due to its ability to direct the development of a Th1 type immune response from naive T cells indicating a critical role in regulating an immune response. Increased plasma levels of IL-12 have been found in neurological disorders and a significant elevations have been measured in patients with multiple sclerosis, autistic, rheumatoid arthritis, seronegative spondylarthropathies and osteoarthritis.
The standard used in this ELISA assay is recombinant human IL-12 p70 that has a total molecular mass of 75 KDa.
Interleukin-12 ELISA kit can be used to analyse in vitro quantitative levels of interleukin-12 p70 (IL-12 p70) in human serum and cell culture supernatant. This assay has a minimum detection limit of < 2.0 pg/ml.
The minimum detection sensitivity level of interleukin-12 (IL-12 p70) using this human interleukin-12 ELISA kit was approximately 2.0 pg/ml. The dynamic assay range for this kit is 7.8 – 500 pg/ml.
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- Full Name: Interleukin-12 (IL-12 P70) ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Cell Culture Supernatant
- Sensitivity: < 2.0 pg/ml