Interleukin-12 p40 ELISA kit is designed for measuring in vitro quantitative amounts of interleukin-12 p40 (IL-12 p40) present in human serum, cell culture supernatant and plasma (EDTA, heparin, citrate). This assay has a minimum detection limit of < 2.0 pg/ml.
Interleukin 12 (IL-12) which is also called by the following names cytotoxic lymphocyte maturation factor (CLMF) and natural killer cell stimulatory factor (NKSF), is a 75kDa glycoprotein heterodimer that is made up of two unequal, genetically-unrelated subunits (p35 and p40 subunits). The larger 40-kDa subunit (p40) is a member of the cytokine receptor family whereas the smaller 35-kDa subunit (p35) has been found to be related to IL-6 and GCSF. The p40 subunit is secreted in excess of IL-12p35 and has the ability to associate with p19 (IL-23P19 or IL-23A) in order to form IL-23. There is also sequence similarity shared between the p19 subunit and IL-6 subfamily members including the p35 subunit of IL-12. However, it can only become biologically active when it combines with the p40 subunit of IL-12 to produce a composite cytokine IL-23, whereas p19 subunit alone has no biological activity.
The following interleukin-12 p40 ELISA kit is intended for analysing many diagnostic applications. The human IL-12 p40 subunit has total molecular weight of 34.7 kDa and is made up of 306 amino acid residues in length. There are potentially four N-linked glycosylation sites and 10 cysteine residues. In murine p40 there is approx. 70% sequence homology to human and it contains 313 amino acid residues possessing 11 conserved cysteines and three N-linked glycosylation sites. For both human and mouse p35 and p40, the separate functions of the mature molecules can be attributed to p35 and p40. The preliminary evidence is indicating that p40 is involved in receptor binding whereas p35 is important in signal transduction. This IL-12 p40 ELISA kit offers a sensitivity limit of 2.0 pg/ml and it can be used to measure human samples of plasma, serum and cell culture supernatant.
The minimum detection sensitivity level of IL-12 p40 (interleukin-12 p40) using current human interleukin-12 p40 ELISA kit was approximately 2.0 pg/ml. The dynamic range for this assay is 31.2 – 2,000 pg/ml.
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- Full Name: Interleukin-12 P40 (IL-12 P40) ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma, Cell Culture Supernatant
- Sensitivity: < 2.0 pg/ml