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Interleukin-12 (IL-12 P40) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin 12 (IL-12) which is also called by the following names cytotoxic lymphocyte maturation factor (CLMF) and natural killer cell stimulatory factor (NKSF), is a 75kDa glycoprotein heterodimer that is made up of two unequal, genetically-unrelated subunits (p35 and p40 subunits). The larger 40-kDa subunit (p40) is a member of the cytokine receptor family whereas the smaller 35-kDa subunit (p35) has been found to be related to IL-6 and GCSF. The p40 subunit is secreted in excess of IL-12p35 and has the ability to associate with p19 (IL-23P19 or IL-23A) in order to form IL-23. There is also sequence similarity shared between the p19 subunit and IL-6 subfamily members including the p35 subunit of IL-12. However, it can only become biologically active when it combines with the p40 subunit of IL-12 to produce a composite cytokine IL-23, whereas p19 subunit alone has no biological activity.

The human IL-12 p40 subunit has total molecular weight of 34.7 kDa and is made up of 306 amino acid residues in length. There are potentially four N-linked glycosylation sites and 10 cysteine residues. In murine p40 there is approx. 70% sequence homology to human and it contains 313 amino acid residues possessing 11 conserved cysteines and three N-linked glycosylation sites. For both human and mouse p35 and p40, the separate functions of the mature molecules can be attributed to p35 and p40. The preliminary evidence is indicating that p40 is involved in receptor binding whereas p35 is important in signal transduction.

INTENDED USE

Interleukin-12 ELISA kit is designed for measuring in vitro quantitative amounts of interleukin-12 p40 (IL-12 p40) present in human serum, cell culture supernatant and plasma (EDTA, heparin, citrate). This assay has a minimum detection limit of < 2.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-12 (IL-12 p40) using this human interleukin-12 ELISA kit was approximately 2.0 pg/ml. The dynamic assay range for this kit is 31.2 – 2,000 pg/ml.

REFERENCES

  1. IL-12p40: an inherently agonistic cytokine. Trends Immunol. (2007) 28 (1): 33-8. Review. Cooper A.M. and Khader S.A.
  2. Making many from few: IL-12p40 as a model for the combinatorial assembly of heterodimeric cytokines. Cytokine. (2015) 76 (1): 53-7. Review. Abdi K. and Singh N.J.
  3. The regulation and biological activity of interleukin 12. Leuk Lymphoma. (1998) 29 (5-6): 427-38. Review. Lee S.M., et al.
  4. Does p40-targeted therapy represent a significant evolution in the management of plaque psoriasis? J Eur Acad Dermatol Venereol. (2012) 26 Suppl 5: 2-8. Review. Griffiths C.E. and Girolomoni G.
  5. IL-12: the role of p40 versus p75. Scand J Immunol. (2002) 56 (1): 1-11. Review. Abdi K.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-12 (IL-12 P40) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Cell Culture Supernatant
  • Sensitivity: < 2.0 pg/ml

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