GET A QUOTE

Interleukin-11 (IL-11) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-11 (IL-11) can also be called adipogenesis inhibitory factor (AGIF) or oprelvekin and it belongs to a family of cytokines that are known as GP130. There are many cell and tissue which can produce IL-11 throughout the body, for example lung, connective tissues, thymus, CNS, bone, skin, testis and uterus. One of the main functions of IL-11 is to promote both in vitro and in vivo primary and secondary immune responses which can be responsible for modulating the antigen-specific antibody reactions. Interleukin-11 has the ability to exert its biological activity via IL-11 receptor by utilising the gp130 molecule as the signalling component of its receptor.

IL-11 is a highly conserved precursor protein which has a total molecular mass of 19kDa and is composed of 199 amino acids. The initial 21 amino acids are referred to as the typical leader sequence for secreted proteins. This first 21 amino acid sequence is removed when the 178 amino acid is being released from the cell. The interleukin-11 molecule is thermally stable and also does not contain any cysteine residues. It can be found to display activity in bone metabolism and during the protection or restoration of the gastrointestinal mucosa. Also, human endometrial stromal cells can form biologically active IL-11 which can be responsible for promoting progesterone-induced decidualization. There are a number of reports that indicate abnormal IL-11 concentrations in serum or plasma being related to urogenital infection, rheumatoid arthritis and immune thrombocytopenia. Interleukin-11 has also been linked to aseptic loosening of total hip replacement implants.

INTENDED USE

Interleukin-11 ELISA kit is intended for analysing in vitro quantitative levels of interleukin-11 (IL-11) present in human serum, cell culture supernatant and plasma. This assay has a minimum detection limit of < 4.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-11 (IL-11) using this human interleukin-11 ELISA kit was approximately 4.0 pg/ml. The dynamic assay range for this kit is 4.0 – 1,600 pg/ml.

REFERENCES

  1. The role of IL-11 in immunity and cancer. Cancer Lett. (2016) 373 (2): 156-63. Review. Xu D.H., et al.
  2. Interleukin-11: A Multifunctional Cytokine with Intrinsically Disordered Regions. Cell Biochem Biophys. (2016) 74 (3): 285-96. Review. Permyakov E.A., et al.
  3. Cytokine signalling via gp130 in gastric cancer. Biochim Biophys Acta. (2009) 1793 (11): 1623-33. Review. Howlett M., et al.
  4. IL-11 signaling as a therapeutic target for cancer. Immunotherapy. (2015) 7 (4): 441-53. Review. Putoczki T.L. and Ernst M.
  5. Interleukin-11. A gp130 cytokine. Ann N Y Acad Sci. (1998) 856: 12-21. Review. Trepicchio W.L. and Dorner A.J.
  6. The biology of interleukin 11. Stem Cells. (1993) 11 Suppl 2: 156-62. Review. Neben S. and Turner K.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-11 (IL-11) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Cell Culture Supernatant
  • Sensitivity: < 4.0 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford