- Created on the 12 March, 2017.
Interleukin-10 ELISA kit can be used for determining in vitro quantitative amounts of interleukin-10 (IL-10) in human serum, cell culture supernatant, plasma and many other biological fluids. This assay has a minimum detection limit of < 4.0 pg/ml.
Interleukin-10 (IL-10) is a member of the four a-helix bundle family with a molecular mass of 39 kDa (160 amino acids) and in solution human IL-10 is only functional has a homodimer. It is essentially a pleiotropic cytokine which plays important roles in regulating lymphoid and myeloid cell functions. IL-10 is a potent suppressor of the effector functions of T-cells, macrophages and NK cells since it has the ability to block the synthesis of cytokines and several accessory cell functions of macrophages. Interleukin-10 is found to be expressed by a number of different cells which includes B cells derived from peripheral blood, EBV-transformed B cell lines, Burkitt’s lymphoma, placental trophoblasts, monocytes, bronchial epithelial cells, AIDS lymphomas, certain melanomas and carcinomas.
Human IL-10 gene is found to be located on chromosome 1 and it is often occurring as a single copy within the genome. It displays strong amino acid sequence and DNA sequence homology to the murine IL-10, it also exhibits similarity to the open reading frame present in the Epstein Barr virus genome and BCRF1. This led to implications there is a possibility that IL-10 may also play a role in the host-virus interactions. The immunosuppressive properties that are shown by IL-10 indicate a potential clinical use for IL-10 in suppressing rejections of grafts following organ transplantations. There is a further possibility of it exerting a strong anti-inflammatory activities too. In many diseases for example Leishmania, Schistosoma mansoni, Trypanosoma and Toxoplasma gondii infections the levels of interleukin-10 have been found to be elevated. High concentrations of IL-10 are also found in mycobacterial infections (Mycobacterium leprae, Mycobacterium avium and Mycobacterium tuberculosis) and in retroviral infections inducing immunodeficiency. The following interleukin-10 ELISA kit is ideal for measuring many types of diagnostic applications.
The minimum detection sensitivity level of IL-10 using current human interleukin-10 ELISA kit was approximately 4.0 pg/ml. The dynamic range for this assay is 4.0 – 1,000 pg/ml.
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- Full Name: Interleukin-10 (IL-10) ELISA Kit
- Reactivity: Human
- Sample Type: Plasma, Cell Culture Supernatant, Serum, Other Biological Fluids
- Sensitivity: < 4.0 pg/ml
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