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Interleukin-1 Alpha (IL-1A) ELISA Kit

  • Created on the 12 March, 2017.

BACKGROUND

Interleukin-1 alpha (IL-1α or IL-1a), belongs to the interleukin 1 family which represents biologically active cell-derived mono nuclear proteins that are involved in immune responses and inflammatory reactions. There are currently two distinct human IL-1 species that have been identified IL-1 alpha and IL-1 beta. They are both biologically distinct but distantly related, sharing similarities such as molecular weight, same receptors on target cells and similar biological effects. Also, they are both polypeptidic cytokines that play vital functions in immunologic reactions, tissue repair and inflammation. IL-1α is however predominantly a cell-associated molecule when compared to IL-1β which is a secreted molecule.

IL-1α is an extracellular peptide which is 17kDa in size and its activity has been demonstrated in a number of biological fluids including serum, gingival fluid, urine, cerebrosinal fluid, amniotic fluid, synovial fluid, sputum and bronchoalveolar lavage (BAL) fluid. IL-1 alpha is usually synthesized primarily as a 31 kDa precursor which lacks a signal peptide. This precursor is cleaved through the cysteine protease calpain, leading to the production of 17.5 kDa mature IL-1 molecule. Furthermore, IL-1α can be formed in monocytes and many other cells in the 31 kDa precursor state.  Interleukin-1 alpha has the ability to act on monocytes or macrophages through inducing its own synthesis in addition to the production of IL-6 and TNF. It can also induce the production of GM-CSF, IL-4 from activated T cells, IL-2 receptors and IL-2. IL-1α can increase immunoglobulin synthesis, stimulate B cell proliferation and maturation, induce prostaglandin synthesis in endothelial cells and smooth muscle cells, NK cell activation and LAK production associated with other cytokines, cartilage and calcium resorption in bones and collagenase production in synovial cells. There is also evidence that high levels of IL-1 alpha are associated with rheumatoid arthritis, atherosclerosis, inflammatory bowel disease, insulin-dependent diabetes mellitus, sepsis, acute and chronic myelogenous leukemia.

INTENDED USE

Interleukin-1 alpha ELISA kit is a method for measuring in vitro quantitative levels of interleukin-1 alpha (IL-1α, IL-1a) in human serum, plasma (EDTA, citrate, heparin) and cell culture supernatant samples. This assay has a minimum detection limit of < 0.5 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of interleukin-1 alpha (IL-1α, IL-1a) using this human interleukin-1 alpha ELISA kit was approximately 0.5 pg/ml. The dynamic assay range for this kit is 4.7 – 300 pg/ml.

REFERENCES

  1. Interleukin 1α and the inflammatory process. Nat Immunol. (2016) 17 (8): 906-13. Review. Di Paolo N.C. and Shayakhmetov D.M.
  2. Molecular cloning, overexpression and characterization of human interleukin 1alpha. Biochem Biophys Res Commun. (2007) 360 (3): 604-8. Rajalingam D., et al.
  3. Gene polymorphism of interleukin-1 alpha and beta in keratocystic odontogenic tumors. J Oral Pathol Med. (2012) 41 (9): 697-701. Eshghyar N., et al.
  4. Treating inflammation by blocking interleukin-1 in humans. Semin Immunol. (2013) 25 (6): 469-84. Review. Dinarello C.A. and van der Meer J.W.
  5. Interleukin-1α and brain inflammation. IUBMB Life. (2015) 67 (5): 323-30. Review. Brough D. and Denes A.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-1 Alpha (IL-1A) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (Heparin, EDTA, Citrate), Cell Culture Supernatant
  • Sensitivity: < 0.5 pg/ml

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