- Created on the 13 February, 2017.
Human IFN-gamma ELISA kit is a reliable quantitative procedure for measuring interferon-gamma (IFN-gamma, IFN-γ, IFN-g) in human serum, plasma and cell culture supernatant. This assay has a minimum sensitivity detection limit of 0.03 IU/ml.
Interferon-gamma (IFN-γ or IFN-gamma) which is also called Type II interferon is an inflammatory cytokine that is a homodimeric glycoprotein which contains approx. 21-24 kD subunits. IFN-gamma has been implicated for the development of fibrosis into inflamed tissues, where its production in under a genetic control that is responsible for influencing the development of fibrosis in lung allografts.
Natural killer (NK) cells and CD8 cytotoxic T cells are also able to produce IFN-γ which is essential in controlling microbial pathogens. This supports the argument that IFN-gamma plays a crucial role in host defence against many infections, where IFN-γ is able to activate both human macrophage oxidative metabolism as well as antimicrobial activity. There is also evidence that it possess antiviral activity during its immune-regulatory functions and control of neointima proliferation. The role of IFN-gamma as a disease marker has been investigated in many different pathological situations which include: infections, transplant rejection, autoimmune diseases, allergy and diabetes.
Reagents which are supplied with the IFN-gamma ELISA kit, please following instructions for storage conditions.
1. Microtiter plate: IFN-γ monoclonal antibodies coated wells.
2. Calibrators (0-5).
4. Conjugate Controls 1 and 2.
5. Washing Solution.
6. Concentrated Chromogen.
7. Substrate Buffer.
8. Stop Solution.
The minimum sensitivity detection limit of interferon-gamma (IFN-g, IFN-γ) using current human IFN-gamma ELISA kit was approximately 0.03 IU/ml. The dynamic range for this assay is 1.0 – 30 IU/ml.
- Interferon-gamma: a historical perspective. Cytokine Growth Factor Rev. (2009) 20 (2): 97-113. Review. Billiau A. and Matthys P.
- Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J. Exp. Med. (1983) 158: 670-689. Nathan C.F., et al.
- Clinical implications of interferon-γ genetic and epigenetic variants. Immunology. (2014) 143 (4): 499-511. Review. Smith N.L. and Denning D.W.
- Impact of interferon-γ on hematopoiesis. Blood. (2014) 24 (16): 2479-86. Review. de Bruin A.M., et al.
- Full Name: Interferon-Gamma (IFN-Gamma) ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma, Cell Culture Supernatant
- Sensitivity: 0.03 IU/ml