Intercellular Adhesion Molecule-3 (ICAM-3) ELISA Kit

  • Created on the 2 May, 2017.


Intercellular adhesion molecule-3 (ICAM-3) which can also been called CD50 is a member of the ICAM family and it functions both as an adhesion and a potent signalling molecule. This protein is abundantly and constitutively expressed by all leukocytes, there is also evidence to indicate that it may be a vital ligand for initiating immune response for LFA-1 molecules. It is made up of type I transmembrane glycoproteins, consisting of 2-9 immunoglobulin-like C2-type domains and also has the ability to bind to the leukocyte adhesion LFA-1 protein. Alternative splicing is known to lead to multiple transcript variants being produced which are encoding different isoforms. ICAM-3 can bind to leukocyte adhesion LFA-1 protein (CD18/CD11a) and this can help to facilitate cell-cell interactions which need to happen in the immune system.


Human ICAM-3 ELISA kit is intended for measuring in vitro quantitative amounts of intercellular adhesion molecule-3 (CD50, ICAM-3) in human cell lysates, plasma, serum, biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 0.4 ng/ml.


The minimum detection sensitivity level of ICAM-3 or CD50 using this human ICAM-3 ELISA kit was 0.4 ng/ml. The dynamic assay range for this kit is 0.781 – 50 ng/ml.


  1. Roles of ICAM-3 and CD14 in the recognition and phagocytosis of apoptotic cells by macrophages. Biochem Soc Trans. (1998) 26 (4): 644-9. Gregory C.D., et al.
  2. Apoptotic cell-derived ICAM-3 promotes both macrophage chemoattraction to and tethering of apoptotic cells. Cell Death Differ. (2012) 19 (4): 671-9. Torr E.E., et al.
  3. Role of ICAM-3 in the initial interaction of T lymphocytes and APCs. Nat Immunol. (2002) 3 (2): 159-68. Montoya M.C., et al.
  4. Macrophage recognition of ICAM-3 on apoptotic leukocytes. J Immunol. (1999) 162 (11): 6800-10. Moffatt O.D., et al.


  • Full Name: Intercellular Adhesion Molecule-3 (ICAM-3) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Cell Culture Supernatant, Serum, Other Biological Fluids, Cell Lysates
  • Sensitivity: 0.4 ng/ml



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford