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Intercellular Adhesion Molecule-3 (ICAM-3) ELISA Kit

  • Created on the 2 May, 2017.

BACKGROUND

Intercellular adhesion molecule-3 (ICAM-3) which can also been called CD50 is a member of the ICAM family and it functions both as an adhesion and a potent signalling molecule. This protein is abundantly and constitutively expressed by all leukocytes, there is also evidence to indicate that it may be a vital ligand for initiating immune response for LFA-1 molecules. It is made up of type I transmembrane glycoproteins, consisting of 2-9 immunoglobulin-like C2-type domains and also has the ability to bind to the leukocyte adhesion LFA-1 protein. Alternative splicing is known to lead to multiple transcript variants being produced which are encoding different isoforms. ICAM-3 can bind to leukocyte adhesion LFA-1 protein (CD18/CD11a) and this can help to facilitate cell-cell interactions which need to happen in the immune system.

INTENDED USE

Human ICAM-3 ELISA kit is intended for measuring in vitro quantitative amounts of intercellular adhesion molecule-3 (CD50, ICAM-3) in human cell lysates, plasma, serum, biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 0.4 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of ICAM-3 or CD50 using this human ICAM-3 ELISA kit was 0.4 ng/ml. The dynamic assay range for this kit is 0.781 – 50 ng/ml.

REFERENCES

  1. Roles of ICAM-3 and CD14 in the recognition and phagocytosis of apoptotic cells by macrophages. Biochem Soc Trans. (1998) 26 (4): 644-9. Gregory C.D., et al.
  2. Apoptotic cell-derived ICAM-3 promotes both macrophage chemoattraction to and tethering of apoptotic cells. Cell Death Differ. (2012) 19 (4): 671-9. Torr E.E., et al.
  3. Role of ICAM-3 in the initial interaction of T lymphocytes and APCs. Nat Immunol. (2002) 3 (2): 159-68. Montoya M.C., et al.
  4. Macrophage recognition of ICAM-3 on apoptotic leukocytes. J Immunol. (1999) 162 (11): 6800-10. Moffatt O.D., et al.

ADDITIONAL INFORMATION

  • Full Name: Intercellular Adhesion Molecule-3 (ICAM-3) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Cell Culture Supernatant, Serum, Other Biological Fluids, Cell Lysates
  • Sensitivity: 0.4 ng/ml

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