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Insulinoma-Associated 2 (Anti-IA2) ELISA Kit

  • Created on the 9 February, 2017.

INTENDED USE

Human anti-IA2 ELISA kit is a reliable quantitative procedure for measuring insulinoma-associated 2 (anti-IA2) in human serum samples. This assay has a minimum sensitivity detection limit of 1.25 U/ml.

BACKGROUND

Insulinoma-associated 2 (Anti-IA2) autoantibodies are found to be highly present in individuals that are in the pre-diabetic phase or those with new-onset type 1 diabetes. There is a direct correlation with the rapid progression to overt type 1 diabetes with the presence of autoantibodies to IA2. It is a member of the tyrosine phosphatases protein family and shares sequence homology with the islet cell antigen 512. Found to be localised in the second defined recombinant islet cell antigen and the dense granules of pancreatic beta cells and the second defined recombinant islet cell antigen. The higher frequency of antibodies to IA2 can be explained by the presence of autoantibodies which are directed to the COOH terminus of IA2 and are lacking in the ICA512 molecule.

A combination of assays using IA2 autoantibodies and GAD65 is seen as a highly relevant approach for assessing type 1 diabetes in children and adolescence. It is possible to detect more than 90% of individuals that are at risk for type 1 diabetes by performing a screen for IA2 autoantibodies and GAD65.

REAGENTS SUPPLIED

Reagents which are supplied with the following anti-IA2 ELISA kit, please following instructions for storage conditions.

1. IA-2 Coated Wells: 12 break apart strips of 8 wells (96 in total) in a frame and sealed in foil bag.
2. Negative Control.
3. Calibrators: 7.5, 35, 120, 350, 4000 u/mL (units are NIBSC 97/550).
4. Positive Control (see QC datasheet for concentration range).
5. Reaction Enhancer.
6. Concentrated Wash Solution.
7. IA-2-Biotin.
8. Reconstitution Buffer for IA-2-Biotin.
9. Streptavidin Peroxidase (SA-POD).
10. Diluent for SA-POD.
11. Peroxidase Substrate (TMB).
12. Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of insulinoma-associated 2 using this human anti-IA2 ELISA kit was approximately 1.25 U/ml. The dynamic range for this assay is 7.5 – 4000.0 U/ml.

REFERENCES

  1. Lack of anti-insulin, anti-GAD, and anti-IA2 autoantibodies in primary antiphospholipid syndrome. Rheumatol Int. (2012) 32 (5): 1479-80. de Carvalho J.F. and Caleiro M.T.
  2. Prevalence of type 1 diabetes autoantibodies (GADA, IA2, and IAA) in overweight and obese children. Diabetes Care. (2010) 33 (4): 820-2. Cambuli V.M., et al.

ADDITIONAL INFORMATION

  • Full Name: Insulinoma-Associated 2 (Anti-IA2) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 1.25 U/ml

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I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

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