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IGF-II ELISA Kit

INTENDED USE

Human IGF-II ELISA kit is a reliable quantitative procedure for measuring insulin like growth factor II (IGFII, IGF-II) in human serum, plasma, CSF, saliva, liquor and urine. This assay has a minimum sensitivity detection limit of 0.02 ng/ml.

BACKGROUND

Insulin like growth factor II (IGF II or IGFII) is encoded by the IGF2 gene and is part of a cluster of genes which are located on chromosome 11 the short (p) arm that undergoes genomic imprinting. IGF-II protein has important roles during the growth and development period before birth, the IGF2 gene is found to be very active during the fetal development phase, however, it is much less active after birth. There are many studies which indicate that human IGF-II has the ability in promoting growth and proliferation of a large number of cells that can be found in many different tissues.

The activity of the IGF2 gene is wholly dependent on which parent it was inherited from, where in majority of tissues, only the copy which is inherited from the father is active (often referred to as the paternally inherited copy) whereas, the copy obtained from the mother is usually not active (known as the maternally inherited copy). This type of parent-specific difference for activation of a gene are due to a phenomenon which is called genomic imprinting.

The following human IGF-II ELISA kit is highly specific, sensitive, easy to use and can provide results relatively quickly, there is virtually zero cross reactivity with IGF-I. One of the main uses for IGF-II is during differential diagnostics of malignancies, where it is possible to differentiate by human IGF-II between adrenocortical adenomas and carcinomas. Also, further tumor differentiation and staging between carcinoma and hyperplasia can be improved by using IGF-II measurements in prostate tumors. The IGF-System seem is relevant in many neurodegeneration diseases such as Parkinson’s and Alzheimer’s.

IGF-II ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Divided up in 12 strips with 8 wells separately breakable, coated with anti-human IGF-II antibody.
  • Standards A-E: Concentration 0.45, 1.5, 3, 5.63, 9 ng/ml, made up from lyophilised human IGF-II.
  • Sample Buffer.
  • Control Serum 1 And Control Serum 2.
  • Antibody Conjugate: Consisting of biotinylated anti-IGF-II antibody conjugated to biotin.
  • Enzyme Conjugate: HRP conjugate to streptavidin.
  • Washing Buffer (20x Concentrated).
  • TMB Substrate.
  • Stopping Solution.
  • Sealing Tape: To cover microtiter plate.

SENSITIVITY

The minimum sensitivity detection limit of human insulin like growth factor II (IGF-II, IGFII) using current IGF-II ELISA kit was approximately 0.02 ng/ml. The dynamic range for this assay is 0.45 – 9.0 ng/ml.

ASSAY CHARACTERISTICS

– Insulin Like Growth Factor II (IGF-II): ELISA
– Calibration: Against the International Standard: WHO NINSC 96/538.
– Sensitivity: 0.02 ng/ml
– Intra Assay Variation: 7.06 – 7.22%
– Inter Assay Variation: 3.07 – 6.61%

REFERENCES

  1. Insulin like growth factor II is a useful marker to detect hepatocellular carcinoma? Eur J Intern Med. (2012) 23 (6): e157-61. Morace C., et al.
  2. Genomic imprinting of insulin-like growth factor 2 (IGF-2) in chronic synovitis. Growth Horm IGF Res. (2007) 17 (6): 500-5. Möller B., et al.
  3. Insulin-like growth factor-II: its role in metabolic and endocrine disease. Clin Endocrinol (Oxf). (2014) 80 (6): 773-81. Review. Livingstone C. and Borai A.
  4. The Relationship of Insulin-Like Growth Factor 2 to Fetal Growth and Adiposity. Horm Res Paediatr. (2016) 85 (2): 75-82. Review. Kadakia R. and Josefson J.

ADDITIONAL INFORMATION

  • Full Name: IGF-II ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Saliva, Liquor, Urine
  • Sensitivity: 0.02 ng/ml

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