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Insulin-like Growth Factor-I (IGF-I) ELISA Kit

  • Created on the 7 February, 2017.

BACKGROUND

Insulin-like growth factor-I (IGF-I) has a molecular weight of 7649 daltons and is identical with Somatomedin C (Sm-C). One of its main function is to regulate growth hormone (GH) and nutrition, even though it is produced by specific tissues it can be affected by a multitude of peptide growth factors and tropic hormones. IGFs are known to avidly bind specific binding proteins which are called Insulin-like growth factor binding proteins (IGFBP), which is in contrast to many other peptide hormones. There are seven classes of IGFBPs that can bind either IGF-I or IGF-II with similar affinities, however, many of these show a preference for IGF-II.

This can cause a major problem when measuring IGF-I due to the ability of IGFBP being able to interfere with the assay.  In the past various techniques have being applied in order to physically separate the IGF-I from its binding protein before carry out the assay such as solid-phase extraction, size exclusion chromatography under acidic conditions and acid-ethanol extraction. These methods are time consuming, inconvenient or give incomplete and non-reproducible recoveries.

To avoid all these difficulties, we provide an uncomplicated ELISA assay which does not require any special sample preparation before measurement. It can also be used for the human serum or plasma in cerebrospinal fluid, conditioned media of cell lines or other body fluids. Due to many mammalian species having a high cross reactivity with IGF-I this assay can also be used in cattle, primate, sheep, donkey, pig, guinea pig dog, rabbit and cat. However, this assay kit is not suitable for mouse, rat or chicken samples.

INTENDED USE

Human IGF-I ELISA kit is a reliable quantitative procedure for measuring insulin-like growth factor-I (IGF-I) in human serum and plasma. This assay has a minimum sensitivity detection limit of 0.09 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of insulin-like growth factor-I (IGF-I) using this IGF-I ELISA kit was approximately 0.09 ng/ml. The dynamic assay range for this kit is 2.0 – 50.0 ng/ml.

REFERENCES

  1. Human conditions of insulin-like growth factor-I (IGF-I) deficiency. J Transl Med. (2012) 10: 224. Review. Puche J.E. and Castilla-Cortázar I.
  2. Insulin-like growth factor-I (IGF-I) and clinical nutrition. Clin Sci (Lond). (2013) 125 (6): 265-80. Review. Livingstone C.
  3. Essential roles of growth hormone (GH) and insulin-like growth factor-I (IGF-I) in the liver. Endocr J. (2012)59 (11): 955-62. Review. Takahashi Y.
  4. Autocrine production of IGF-I increases stem cell-mediated neuroprotection. Stem Cells. (2015) 33 (5): 1480-9. Lunn J.S., et al.

ADDITIONAL INFORMATION

  • Full Name: Insulin-like Growth Factor-I  (IGF-I) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.09 ng/ml

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