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IGFBP-2 ELISA Kit

INTENDED USE

Human IGFBP-2 ELISA kit is a reliable quantitative procedure for measuring insulin like growth factor binding protein 2 (IGFBP2, IGFBP-2) in human serum, plasma, CSF, saliva and other body fluids. This assay has a minimum sensitivity detection limit of 0.2 ng/ml.

BACKGROUND

Insulin like growth factor binding protein 2 (IGFBP2) is an unglycosylated 31.3kDa polypetide protein which shows no circadian rhythm in circulation and forms binary IGF-complexes. The IGFBP2 concentration in blood is age-dependent and the serum concentration is found to increase during fasting state, after trauma, after any major surgery and in malignant diseases.

It is most intensive in malignant diseases where the correlation of the IGFBP-2 level to the degree of progression can be a striking feature in a number of different tumour types as is the normalisation of the IGFBP serum levels after remission. During the GH-therapy, for example in GH abuse (doping) and in short stature the human IGFBP-2 levels have been found to decrease. Also, in trisomy 18 IGFBP-2 in maternal serum is decreased and IGFBP-1 is increased, this allows the ratio IGFBP-2 /IGFBP-1 to be a good indicator/marker for chromosome abnormality.

IGFBP-2 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with anti-IGFBP-2 antibody.
  • Standards A-E: Lyophilized, containing human IGFBP-2, concentrations 2, 10, 20, 40, 80 ng/ml.
  • Dilution Buffer.
  • Control Serum (KS1) And Control Serum (KS2): Lyophilized. Contains human IGFBP-2 antibody.
  • Washing Buffer (20x Concentrated).
  • TMB Substrate.
  • Stopping Solution.
  • Sealing Tape: To cover microtiter plate.

SENSITIVITY

The minimum sensitivity detection limit of human insulin like growth factor binding protein 2 (IGFBP-2, IGFBP2) using current IGFBP-2 ELISA kit was approximately 0.02 ng/ml. The dynamic range for this assay is 2.0 – 80.0 ng/ml.

ASSAY CHARACTERISTICS

– Insulin Like Growth Factor 2 (IGFBP-2, IGFBP2): ELISA
– Analytical Sensitivity: 0.2 ng/ml
– Interference: None
– Recovery: 100 – 112%
– Intra Assay Variation: 137 – 956 ng/ml
– Inter Assay Variation: 311 – 698 ng/ml
– Linearity Of Dilution: 415 – 1055 ng/ml
– Specificity: Highly specific for human IGFBP-2, low cross reaction with cat, dog, goat, donkey and horse. No cross reaction with mouse, rat, guinea pig, rabbit, chicken, pig and sheep. Also, no cross reaction with IGFBP-1 or IGFBP-3.

REFERENCES

  1. IGFBP-2: The dark horse in metabolism and cancer. Cytokine Growth Factor Rev. (2015) 26 (3): 329-46. Review. Russo V.C., et al.
  2. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol. (2014) 58: 154-8. Sunderić M., Mihailović N. and Nedić O.
  3. Insulin like growth factor binding protein 2 as a novel biomarker for disease activity and renal pathology changes in lupus nephritis. Clin Exp Immunol. (2016) 184 (1): 11-8. Ding H., et al.
  4. IGFBP-2 directly stimulates osteoblast differentiation. J Bone Miner Res. (2014) 29 (11): 2427-38. Xi G., et al.

ADDITIONAL INFORMATION

  • Full Name: IGFBP-2 ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Saliva, CSF, Body Fluids
  • Sensitivity: 0.2 ng/ml

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