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IGFBP-1 ELISA Kit

INTENDED USE

Human IGFBP-1 ELISA kit is a reliable quantitative procedure for measuring insulin like growth factor 1 (IGFBP1, IGFBP-1) in human serum, plasma, body fluids, tissue lysates and cell culture supernatant. This assay has a minimum sensitivity detection limit of < 1.0 pg/ml.

BACKGROUND

Insulin like growth factor binding protein 1 (IGFBP-1 or IBP-1) is also called by many other names which include placental protein-12, growth hormone independent binding protein, binding protein-25, 26 and 28, amniotic fluid binding protein(AFBP) and alpha-pregnancy-associated endometrial globulin. It is a low molecular weight protein that is synthesised by the liver, secretory endometrium or decidua. The standard used in the following human IGFBP-1 ELISA kit is a recombinant human IGFBP1 that is made up of 234 amino acids with a total molecular mass of 25KDa.

There is also evidence that it is present in human serum. IGFBP-1 has the ability to bind with high affinity to insulin like growth factors IGF 1 and IGF 2, binding of this protein can prolong the half-life of IGFs as well as altering their interaction with cell surface receptors. IGFBP1 is described as a paracrine regulator for cell growth.

IGF binding proteins are known to expressed at a number of different concentrations, in many cells and have vital roles in regulating the activity of IGF I and II. The IGF binding protein gene is organized in four exons and spans 5.9 kb, it is a single copy gene that is located on chromosome 7.

IGFBP-1 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter plate: Coated with mouse IGFBP-1 antigen. Wells are separately breakable.
  • Standards A-G: Lyophilized, native human IGFBP-1, concentration 0-2,000 ng/ml.
  • Control Serum 1 and Control Serum 2: Lyophilised anti human IGFBP-1, human serum.
  • Antibody Conjugate: Mouse biotinylated anti human IGFBP-1 antibody.
  • Enzyme Conjugate: HRP-labelled streptavidin.
  • Dilution Buffer.
  • Washing Buffer: 20-fold concentrated solution.
  • Substrate S: HRP substrate.
  • Stopping Solution: 0.2 M sulphuric acid.
  • Sealing Tape: To cover microtiter plate.
  • Instructions.
  • Quality Control Certificate.

SENSITIVITY

The minimum sensitivity detection limit of insulin like growth factor 1 (IGFBP-1, IGFBP1) using current human IGFBP-1 ELISA kit was approximately 1.0 pg/ml. The dynamic range for this assay is 31.2 – 2,000.0 ng/ml.

REFERENCES

  1. Insulin like growth factor binding protein 1: recent findings and new directions. Proc Soc Exp Biol Med. (1997) 216 (3): 319-57. Review. Lee P.D., et al.
  2. The gene for insulin-like growth factor binding protein-1 is localized to human chromosomal region 7p14-p12. Genomics (1990) 6: 413-418. Ekstrand, J., et al.
  3. Organization of the gene encoding the insulin-like growth factor binding protein IBP-1. Biochem. Biophys. Res. Commun. (1988) 157: 898-907. Brinkman, A., et al.
  4. Multifaceted roles for IGFBP-1 in human endometrium during implantation and pregnancy. Ann N Y Acad Sci. (1997) 828: 146-56. Review. Giudice LC.
  5. Regulation of IGFBP-1 in humans. Prog Growth Factor Res. (1995) 6 (2-4): 449-56. Review. Brismar K., Hilding A. and Lindgren B.

ADDITIONAL INFORMATION

  • Full Name: IGFBP-1 ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Cell Culture Supernatant, Body Fluids
  • Sensitivity: <1.0 pg/ml

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