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Insulin ELISA Kit (Rat)

  • Created on the 29 March, 2018.

BACKGROUND

Insulin is a peptide hormone which is produced and secreted by the beta cells found in the pancreatic islets. It is important for regulating metabolism of carbohydrates, proteins and fats by promoting the absorption of glucose from the blood into liver, skeletal muscle and fat cells. This glucose is able to serve as a form of energy to various cells or it be converted into fat so that it can be used at a later point. Insulin is also known to affect many other metabolic processes for example the breakdown of proteins and fats. Diabetes is one of the most common problem that has been linked to insulin, this is known to occur in individuals where the body is unable to secrete enough insulin or when the body is unable to utilise the secreted insulin effectively.

INTENDED USE

Rat Insulin ELISA kit can be used for quantitative analysis of rat insulin levels using serum or plasma. This assay has a minimum sensitivity detection limit of 0.01 ng/ml.

SENSITIVITY

The standard range of rat insulin using this rat insulin ELISA kit was approximately 0.01 ng/ml. The dynamic assay range for this kit is 0.156 – 10 ng/ml.

REFERENCES

  1. A quantitative enzyme-linked immunosorbent assay for rat insulin. J Immunoassay. (1988) 9 (2): 135-58. Bank H.L.
  2. Insulin therapy normalizes reduced myocardial β-adrenoceptors at both the onset and after sustained hyperglycemia in diabetic rats. Life Sci. (2015) 132: 101-7. Haley J.M., et al.
  3. Development of an ultrasensitive immunoassay using affinity maturated antibodies for the measurement of rodent insulin. Anal Biochem. (2015) 473: 72-9. Imai S., et al.
  4. Long-term treatment with atrial natriuretic peptide inhibits ATP production and insulin secretion in rat pancreatic islets. Am J Physiol Endocrinol Metab. (2011) 300 (3): E435-44. You H. and Laychock S.G.

ADDITIONAL INFORMATION

  • Full Name: Insulin ELISA Kit (Rat)
  • Reactivity: Rat
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.01 ng/ml

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford