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Insulin Ab ELISA Kit

  • Created on the 12 June, 2017.

BACKGROUND

Diabetes-related autoantibodies are found to be linked to type 1 diabetes and this is a condition which is characterised by limited or in some cases fully missing secretion of insulin. Many morphological investigations have demonstrated that this process reflects the destruction of beta cells, leading to the loss of beta cell function and thereby causing inadequate production of insulin. This is a feature of type 1 diabetes but it is thought not to be the cause of it. The detection of anti-insulin autoantibodies may not be possible until after several months or in some rare cases many years following the onset of a full clinical manifestation of the diseases. These autoantibodies are causing insulin depot formation which is able to simulate a resistance against any animal insulin hormonal treatment. IgG antibodies are the most common while IgE antibodies have been found to result in causing insulin allergy, at high titers, IgG antibodies are able to limit insulin action that may diminish or delay action of insulin. The development of antibodies is largely dependent of the molecular structure, purity and storage conditions of the insulin administered as well as patient factors (such as age, HLA type, delivery route). Four common diabetes-related autoantibody tests include: Insulin Autoantibodies (IAA), Glutamic Acid Decarboxylase Autoantibodies (GADA), Insulinoma-Associated-2 Autoantibodies (IA-2A) and Islet Cell Cytoplasmic Autoantibodies (ICA).

INTENDED USE

Human insulin ELISA kit is a protocol for analysing in-vitro quantitative levels of IgG antibodies to insulin in human serum and plasma. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

Mixture of highly purified preparations of porcine, bovine and recombinant human insulin are bound to the microwells.

SENSITIVITY

The minimum detection sensitivity level of human insulin IgG antibodies using this human insulin ELISA kit was 0.5 U/ml. The dynamic assay range for this kit is 6.3 – 100.0 U/ml.

REFERENCES

  1. The anti-insulin trimolecular complex in type 1 diabetes. Curr Opin Endocrinol Diabetes Obes. (2010) 17 (4): 329-34. Review. Michels A.W. and Nakayama M.
  2. Clinical significance of insulin antibodies in insulin-treated diabetic patients. Diabetes Care. (1989) 12 (9): 641-8. Review. Van Haeften T.W.
  3. Autoimmune insulin syndrome. Biomed Pharmacother. (1989) 43 (8): 581-6. Review. Archambeaud-Mouveroux F., et al.
  4. Insulin assays: previously known and new analytical features. Clin Lab. (2003) 49 (3-4): 113-21. Review. Sapin R.
  5. Role of insulin in glucose-stimulated insulin secretion in beta cells. Curr Diabetes Rev. (2005) 1 (3): 309-30. Review. Goren H.J.
  6. Allergic reactions to human insulin: a review of current knowledge and treatment options. Endocrine. (2010) 37 (1): 33-9. Review. Akinci B., et al.

ADDITIONAL INFORMATION

  • Full Name: Insulin Ab ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.5 U/ml

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Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford