Human influenza A IgG ELISA kit is designed for detecting in-vitro quantitative amounts of human IgG antibodies to influenza A (influenza-A IgG) in serum and plasma samples. This assay has a minimum analytical sensitivity limit of 1.1 U/ml.
There are four different types of influenza viruses (A, B, C and D) and only types A and B are responsible for causing human diseases. In general, wild aquatic birds are the natural host for majority of influenza A type viruses (such as swans, geese, ducks, shorebirds and gulls). Influenza A viruses can be divided into subtypes, either hemagglutinin (HA) or neuraminidase (NA). Currently, there are 18 HA subtypes and 11 NA subtypes which have been identified, also it is possible to have a large number of different combinations of HA and NA proteins. However, only two influenza A virus subtypes (for example H1N1 and H3N2) are presently found to be in general circulation among people. Influenza viruses belonging to the family Orthomyxoviridae and also known as the flu.
INFLUENZA A IGG ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Calibrator A (Negative Control).
- Calibrator D (Positive Control).
- Calibrator B (Cut-Off Standard).
- Calibrator C (Weak Positive Control).
- Sample Diluent.
- Enzyme Conjugate.
- TMB Substrate.
- Washing Buffer (10x).
- Stop Solution.
- Influenza A antigen coated microtiter strips.
The minimum detection sensitivity level IgG to to influenza A (influenza A IgG) using current human influenza A-IgG ELISA kit was 1.1 U/ml. The dynamic assay range for this kit is 1.0 – 150.0 U/ml.
– Influenza A ELISA: IgG
– Intra-Assay-Precision: 8.5 %
– Inter-Assay-Precision: 6.5 %
– Inter-Lot-Precision: 3.8 – 6.1 %
– Analytical Sensitivity: 1.09 U/mL
– Recovery: 100 – 114 %
– Linearity: 79 – 114 %
– Cross-Reactivity: No cross-reactivity to RSV, Adenovirus and Parainfluenza 1/2/3.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 88 %
– Clinical Sensitivity: 100 %
- A pilot study showing differences in glycosylation patterns of IgG subclasses induced by pneumococcal, meningococcal, and two types of influenza vaccines. Immun Inflamm Dis. (2014) 2 (2): 76-91. Vestrheim A.C., et al.
- Detection of influenza A/H1N1-specific human IgG-secreting B cells in older adults by ELISPOT assay. Viral Immunol. (2014) 27 (2): 32-8. Painter S.D., et al.
- Influenza A and B virus IgG and IgM serology by enzyme immunoassays. Epidemiol Infect. (1987) 99 (1): 55-64. Koskinen P., et al.
- Serum IgG subclass responses of humansto inactivated and live influenza A vaccines compared to natural infections with influenza A. J Med Virol. (1990) 30 (2): 92-6. Hocart M.J., et al.
- Full Name: Influenza A IgG ELISA Kit
- Reactivity: Human
- Sample Type: Plasma, Serum
- Sensitivity: 1.1 U/ml