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IGFBP–3 ELISA Kit (Rat/Mouse)

  • Created on the 28 March, 2018.

BACKGROUND

IGFBP-3 is the major carrier of somatomedin C (also known as IGF-1), it is made up of stable complexes consisting of either IGF-1, IGF-2 or ALS (acid labile subunit). These growth factors can then be transported through the blood stream. The blood levels of these proteins can be regulated by human growth hormone (hGH). It has been found that the concentration of IGFBP-3 is the greatest during puberty and childhood and then it decreases during adulthood. The protein has been found to exert anti-proliferative effect on a number of cell types through blocking the ability of IGF-1 or IGF-2 to activate the IGF1 receptor. Evidence from cell growth experiments, epidemiological and cancer models indicates that IGFBP-3 might be functioning as a low penetrance tumor suppressor gene.

INTENDED USE

Rat/Mouse IGBPF-3 ELISA kit is intended for detecting quantitative amounts of rat or mouse insulin-like growth factor binding protein-3 (IGFBP-3) using serum, plasma and cell culture media samples. This assay has a minimum sensitivity detection limit of 0.09 ng/ml.

SENSITIVITY

The standard range of mouse and rat insulin-like growth factor binding protein-3 (IGFBP-3) using this rat/mouse IGFBP-3 ELISA kit was approximately 0.09 ng/ml. The dynamic assay range for this kit is 0.039 – 25 ng/ml.

REFERENCES

  1. Recombinant IGFBP-3 inhibits allergic lung inflammation, VEGF production, and vascular leak in a mouse model of asthma. Allergy. (2012) 67 (7): 869-77. Kim S.R., et al.
  2.  IGFBP-3 is a metastasis suppression gene in prostate cancer. Cancer Res. (2011) 71 (15): 5154-63. Mehta H.H., et al.
  3. Competitive binding assay for determination of rat insulin-like growth factor binding protein-3. Endocrinology. (1998) 139 (3): 1454-7. Frystyk J. and Baxter R.C.
  4. IGF-I and IGFBP-3 transport in the rat heart. Am J Physiol Endocrinol Metab. (2003) 284 (1): E237-9. Boes M., et al.
  5. IGFBP-3 inhibits the proliferation of neural progenitor cells. Neurochem Res. (2011) 36 (3): 406-11. Kalluri H.S. and Dempsey R.J.

ADDITIONAL INFORMATION

  • Full Name: IGFBP-3 ELISA Kit (Rat/Mouse)
  • Reactivity: Mouse, Rat
  • Sample Type: Serum, Plasma, Cell Culture Media
  • Sensitivity: 0.09 ng/ml

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L. Johnston
PhD Student / University of Glasgow

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Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

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Purchasing / University of Oxford