Rat/Mouse IGF-I ELISA Kit (Insulin-Like Growth Factor-I)

Full Name: Rat/Mouse IGF-I ELISA Kit (Insulin-Like Growth Factor-I)
Reactivity: Mouse, Rat
Sample Type: Serum, Plasma
Sensitivity: < 0.029 ng/ml

BACKGROUND

Somatomedin C and insulin-like growth factor I (IGFI, IGF-I) are similar molecules having a molecular weight of 7649 daltons (Sm-C). Although growth hormone (GH) is created by certain tissues, it can still be influenced by a variety of peptide growth factors and tropic hormones. In a healthy person, GH is not markedly elevated because the body suppresses GH secretion when it senses an abundance of somatomedin C. In cancer patients, the stimulation of somatomedin C and/or IGF-I production can create an increase in GH secretion. Hence, tumor cells secrete growth factors that can stimulate muscle cell synthesis and indirectly promote tumor growth by stimulating growth hormone release. In contrast to many other peptide hormones, IGFs are known to ardently bind particular binding proteins known as Insulin-like growth factor binding proteins (IGFBP).

There are seven different classes of IGFBPs and while they can bind IGF-I or IGF-II with identical affinities, many of them prefer IGF-II. This allows for an exchange of IGF-I with IGF-II in a feedback mechanism to ensure that these actions are not misaligned. The primary function of the growth hormone is to promote and regulate the growth of all body tissues. This includes growth during childhood and adolescence, as well as during a pregnancy, lactation, or after trauma such as surgery.

This can be a serious issue when attempting to measure IGF-I because IGFBP has the potential to interfere with the assay. Before performing the assay, a variety of procedures have been used to physically separate the IGF-I from its binding protein, including solid-phase extraction, size exclusion chromatography in an acidic environment and acid-ethanol extraction. Some techniques take a lot of time, are inconvenient or result in inaccurate and erratic recoveries.

INTENDED USE

Rat/Mouse IGF-I ELISA kit can be used for measuring quantitative levels of mouse or rat insulin-like growth factor-I (IGFI, IGF-I) using serum and plasma samples. This assay has a minimum sensitivity detection limit of < 0.029 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Coated with anti-IGF-I antibody.
• Standards A-E: Lyophilised, concentrations are between 0.5 – 18 ng/ml (0.5, 2.5, 6, 12 und 18 ng/ml).
• Sample Buffer.
• Control Sera KS1 & KS2.
• Antibody Conjugate.
• Enzyme Conjugate.
• Washing Buffer (20x Concentrated).
• Substrate.
• Stopping Solution.
• Sealing Tape

SENSITIVITY

The standard range of rat or mouse insulin-like growth factor-I (IGF-I) using current rat/mouse IGF-I ELISA kit was approximately 0.029 ng/ml. The dynamic range for this assay is 0.5 – 18.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: < 0.029 ng/Ml
– Inter Assay Variability: 5.3 – 6.72%
– Intra Assay Variability: 4.2 – 5.9%

REFERENCES

1. Aging, atherosclerosis, and IGF-I. J Gerontol A Biol Sci Med Sci. (2012) 67 (6): 626-39. Review. Higashi Y., et al
2. Serum IGF-I_levels correlate negatively to liver damage in diabetic rats. Biotech Histochem. (2013) 88 (3-4): 194-201. Aksu I., et al
3. The emerging role of IGF-I-deficiency in cardiovascular aging: recent advances. J Gerontol A Biol Sci Med Sci. (2012) 67 (6): 599-610. Review. Ungvari Z. and Csiszar A.
4. Regulation of IGF-I/PI3K/Akt signalling by the phosphoinositide phosphatase pharbin. J Biochem. (2011) 150 (1): 83-93. Wang F., et al.

OTHER RELATED ELISA KITS

• Insulin ELISA Kit (Rat)
• IGFBP-2 ELISA Kit (Rat/Mouse)
• IGFBP-3 ELISA Kit (Rat/Mouse)