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IGF–I ELISA Kit (Rat/Mouse)

  • Created on the 28 March, 2018.

BACKGROUND

Insulin-like growth factor 1 (IGF-1) is also referred to as somatomedin C, it is a sulfaction factor which has a similar molecular structure as insulin and it also possesses non-suppressible insulin-like activity. IGF-1 has a molecular weight of 7,649 Da, containing a single chain of 70 amino acids that is held together by three intracellular disulphide bridges. It is mainly produced as an endocrine hormone through the liver and has the ability to target specific tissues in either an autocrine or a paracrine manner. IGF-1 is the predominate mediator of growth hormone effects, which are found to be mediated via IGF1R receptor, leading to the activation of the AKT signalling pathway. This can result in the stimulation of cell growth and proliferation, it is a potent inhibitor of programmed cell death.

INTENDED USE

Rat/Mouse IGF-1 ELISA kit can be used for measuring quantitative levels of mouse or rat insulin-like growth factor-1 (IGF-1) using serum and plasma samples. This assay has a minimum sensitivity detection limit of < 0.029 ng/ml.

SENSITIVITY

The standard range of rat or mouse insulin-like growth factor-1 (IGF-1) using this rat/mouse IGF-1 ELISA kit was approximately 0.029 ng/ml. The dynamic assay range for this kit is 0.5 – 18.0 ng/ml.

REFERENCES

  1. Aging, atherosclerosis, and IGF-1. J Gerontol A Biol Sci Med Sci. (2012) 67 (6): 626-39. Review. Higashi Y., et al
  2. Serum IGF-1 levels correlate negatively to liver damage in diabetic rats. Biotech Histochem. (2013) 88 (3-4): 194-201. Aksu I., et al
  3. The emerging role of IGF-1 deficiency in cardiovascular aging: recent advances. J Gerontol A Biol Sci Med Sci. (2012) 67 (6): 599-610. Review. Ungvari Z. and Csiszar A.
  4. Regulation of IGF-1/PI3K/Akt signalling by the phosphoinositide phosphatase pharbin. J Biochem. (2011) 150 (1): 83-93. Wang F., et al.

ADDITIONAL INFORMATION

  • Full Name: IGF-1 ELISA Kit (Rat/Mouse)
  • Reactivity: Mouse, Rat
  • Sample Type: Serum, Plasma
  • Sensitivity: < 0.029 ng/ml

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Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

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Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

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Lead Scientists / AstraZeneca

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Purchasing / University of Oxford