Human Growth Hormone (hGH) ELISA Kit

  • Created on the 6 February, 2017.


Human growth hormone ELISA kit is a reliable quantitative procedure for measuring growth hormone (hGH) in human serum and plasma. This assay has a minimum sensitivity detection limit of 0.2 µIU/ml.


Growth hormone is a polypeptide hormone with a molecular weight 21,500 Da that is produced by the acidophil cells of the anterior pituitary and is responsible for stimulating growth and cell reproduction in humans and other vertebrate animals. hGH is released into the bloodstream from the pituitary in a pulsatile manner that is under the regulatory control of GH-releasing factor (GHRF) and hypothalamic somatostatin (SS). There are many stimulators of GH secretion which include exercise, fasting, stress, dietary protein, deep sleep, estradiol and hypoglycaemia. There are also inhibitors of GH secretion and these include glucocorticoids, cortisol, free fatty acids and dietary carbohydrates.

One of the main physiological functions of human growth hormone is to act on the liver and other tissues in order to produce somatomedins, this is able to in turn induce growth by direct action on the target tissues. HGH can exerts its effect directly on target organs such as muscles, cartilage and bones or indirectly through the release of insulin-like growth factor (IGF) that is produced in the liver. Unlike hGH which in adults can average about 5 peaks lasting 10-30 minutes a day, the concentration of somatomedin present in serum is kept stable by virtue of being largely bound to circulating plasma proteins.

The measurement of serum hGH using the following growth hormone ELISA kit is useful for:
(a). Index of pituitary function when hypopituitarism (either idiopathic or due to tumour and surgery) is suspected.
(b). Determination and treatment of growth retardation, hGH hyposecretion is one of the various causes that can lead to small stature in children. Measuring serum hGH concentrations, especially after a provocative stimulus (absence of response), is an important way to establish the diagnosis of this condition, it is also possible to treat these group of patients by hGH administration.
(c). Diagnosing and treating gigantism and acromegaly, especially after provocative inhibitory test (absence of response), can be used to establish the diagnosis of hGH hypersecretion which can be due to acidophilic pituitary tumour. This is known to cause gigantism in children and acromegaly in adults, it is also possible to treat both of these disorders by either surgery or radiation.


The minimum sensitivity detection limit of hGH using current human growth hormone ELISA kit was approximately 0.2 µIU/ml. The dynamic range for this assay is 0.64 – 74.0 µIU/ml.


  1. Acta Paediatr (1996) 125: 399. Lewis U.J., et al.
  2. Horm. Res. (1993) 40: 168. Chevenne D., et al.
  3. J Endocrinol (1992) 133: 447. Dattani M.T., et al.
  4. Scand J Clin Lab Invest (1998) 58 (5): 373. Fisker S., et al.
  5. J Clin Endocrinol Metab (1996) 81:2460-2467. Van der Berg G.


  • Full Name: Human Growth Hormone (hGH) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.2 µIU/ml



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford