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Human Growth Hormone (hGH) ELISA Kit

  • Created on the 6 February, 2017.

BACKGROUND

Growth hormone is a polypeptide hormone with a molecular weight 21,500 Da that is produced by the acidophil cells of the anterior pituitary and is responsible for stimulating growth and cell reproduction in humans and other vertebrate animals. hGH is released into the bloodstream from the pituitary in a pulsatile manner that is under the regulatory control of GH-releasing factor (GHRF) and hypothalamic somatostatin (SS). There are many stimulators of GH secretion which include exercise, fasting, stress, dietary protein, deep sleep, estradiol and hypoglycaemia. There are also inhibitors of GH secretion and these include glucocorticoids, cortisol, free fatty acids and dietary carbohydrates.

One of the main physiological functions of human growth hormone is to act on the liver and other tissues in order to produce somatomedins, this is able to in turn induce growth by direct action on the target tissues. HGH can exerts its effect directly on target organs such as muscles, cartilage and bones or indirectly through the release of insulin-like growth factor (IGF) that is produced in the liver. Unlike hGH which in adults can average about 5 peaks lasting 10-30 minutes a day, the concentration of somatomedin present in serum is kept stable by virtue of being largely bound to circulating plasma proteins.

The measurement of serum hGH is useful for:
(a). Index of pituitary function when hypopituitarism (either idiopathic or due to tumour and surgery) is suspected.
(b). Determination and treatment of growth retardation, hGH hyposecretion is one of the various causes that can lead to small stature in children. Measuring serum hGH using highly sensitive ELISA assays, especially after a provocative stimulus (absence of response), is an important way to establish the diagnosis of this condition, it is also possible to treat these group of patients by hGH administration.
(c). Diagnosing and treating gigantism and acromegaly, especially after provocative inhibitory test (absence of response), can be used to establish the diagnosis of hGH hypersecretion which can be due to acidophilic pituitary tumour. This is known to cause gigantism in children and acromegaly in adults, it is also possible to treat both of these disorders by either surgery or radiation.

INTENDED USE

Human growth hormone ELISA kit is a reliable quantitative procedure for measuring growth hormone (hGH) in human serum and plasma. This assay has a minimum sensitivity detection limit of 0.2 µIU/ml.

SENSITIVITY

The minimum sensitivity detection limit of growth hormone (hGH) using this human growth hormone ELISA kit was approximately 0.2 µIU/ml. The dynamic assay range for this kit is 0.64 – 74.0 µIU/ml.

REFERENCES

  1. Acta Paediatr (1996) 125: 399. Lewis U.J., et al.
  2. Horm. Res. (1993) 40: 168. Chevenne D., et al.
  3. J Endocrinol (1992) 133: 447. Dattani M.T., et al.
  4. Scand J Clin Lab Invest (1998) 58 (5): 373. Fisker S., et al.
  5. J Clin Endocrinol Metab (1996) 81:2460-2467. Van der Berg G.

ADDITIONAL INFORMATION

  • Full Name: Human Growth Hormone (hGH) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.2 µIU/ml

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