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Human Growth Hormone (hGH) ELISA Kit

INTENDED USE

Human growth hormone ELISA kit is a reliable quantitative procedure for measuring hGH (human growth hormone, human GH) in serum and plasma. This assay has a minimum sensitivity detection limit of 0.2 µIU/ml.

BACKGROUND

Growth hormone (GH) is a polypeptide hormone with a molecular weight 21,500 Da that is produced by the acidophil cells of the anterior pituitary and is responsible for stimulating growth and cell reproduction in humans and other vertebrate animals. Human growth hormone is released into the bloodstream from the pituitary in a pulsatile manner that is under the regulatory control of GH-releasing factor (GHRF) and hypothalamic somatostatin (SS). There are many stimulators of hGH secretion which include exercise, fasting, stress, dietary protein, deep sleep, estradiol and hypoglycaemia. There are also inhibitors of GH secretion and these include glucocorticoids, cortisol, free fatty acids and dietary carbohydrates. This hGH ELISA kit is designed to be used for analysing serum and plasma samples.

One of the main physiological functions of hGH is to act on the liver and other tissues in order to produce somatomedins, this is able to in turn induce growth by direct action on the target tissues. Human growth hormone can exerts its effect directly on target organs such as muscles, cartilage and bones or indirectly through the release of insulin-like growth factor (IGF) that is produced in the liver. Unlike hGH which in adults can average about 5 peaks lasting 10-30 minutes a day, the concentration of somatomedin present in serum is kept stable by virtue of being largely bound to circulating plasma proteins.

The measurement of serum human growth hormone (hGH)can be useful for:
(a). Index of pituitary function when hypopituitarism (either idiopathic or due to tumour and surgery) is suspected.
(b). Determination and treatment of growth retardation, hGH hyposecretion is one of the various causes that can lead to small stature in children. Measuring serum hGH concentrations, especially after a provocative stimulus (absence of response), is an important way to establish the diagnosis of this condition, it is also possible to treat these group of patients by hGH administration.
(c). Diagnosing and treating gigantism and acromegaly, especially after provocative inhibitory test (absence of response), can be used to establish the diagnosis of hGH hypersecretion which can be due to acidophilic pituitary tumour. This is known to cause gigantism in children and acromegaly in adults, it is also possible to treat both of these disorders by either surgery or radiation.

HGH ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Breakable Microtiter Plate: Wells pre-coated with anti human GH monoclonal antibodies.
  • Conjugate: Consisting of HRP labelled monoclonal anti-GH antibody in stabilizing buffer.
  • Conjugate Buffer.
  • Zero Calibrator.
  • Calibrators (1-5): Concentration 0.45, 5.4, 12.90, 43.50, 98.0 µIU/ml, made up from anti hGH.
  • Wash Solution (200x).
  • Controls 1 and Control 2: Contains anti human GH antibody.
  • TMB Chromogen Solution.
  • Stop Solution: HCl 1N.

SENSITIVITY

The minimum sensitivity detection limit of human growth hormone (hGH) using current hGH ELISA kit was approximately 0.2 µIU/ml. The dynamic range for this assay is 0.76 – 71.5 µIU/ml.

ASSAY CHARACTERISTICS

– Human Growth Hormone (hGH): ELISA
– Detection Limit: 0.17 µIU/ml
– Specificity (Cross Reactivity): CG 100000 mIU/ml (1.9 – 13.5 µIU/ml), hPL 10000 ng/ml (1.4 – 13.0 µIU/ml), PRL 12500 ng/ml (1.8 – 12.6 µIU/ml)
– Intra-Assay Variability: 4.9 – 5.4%
– Inter-Assay Variability: 5.1 – 8.1%
– Recovery: 94 – 113%
– Hook Effect: None hook effect up to 4000 µIU/ml of hGH.

REFERENCES

  1. Human growth hormone therapy of non-growth hormone deficient children. Pediatrician. (1987) 14 (3): 178-82. Ranke MB.
  2. Growth hormone therapy: emerging dilemmas. Pediatr Endocrinol Rev. (2011) 8 (4): 364-73. Laron Z.
  3. Monoclonal antibodies to human growth hormone modulate its biological properties. Mol Immunol. 1995 32 (6): 399-405. Roguin LP et al.
  4. Structure and properties of members of the hGH family: a review. Endocr J. (2000) 47 Suppl: S1-8. Lewis UJ, et al.

ADDITIONAL INFORMATION

  • Full Name: Human Growth Hormone (hGH) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.2 µIU/ml

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