- Created on the 15 February, 2017.
hCG ELISA kit is a reliable quantitative procedure for measuring human chorionic gonadotropin (hCG) in serum and plasma. This assay has a minimum sensitivity detection limit of < 5.0 mIU/ml.
Chorionic gonadotropin (hCG) is a 38.4 kD glycoprotein hormone that is usually secreted by the placenta during pregnancy. Structurally it is composed of two subunits, alpha and beta, where the alpha subunit is identical to that found in FSH, TSH and LH glycoprotein hormones and the beta subunit is the specific portion and this in known to differ from one hormone to another. The difference in the beta subunit accounts for the immunochemical distinctiveness and the biological specificity of each hormone.
After conception, the concentration of hCG is rapidly elevated until it reaches a peak towards the end of the first trimester. This high concentration is then maintained throughout pregnancy. After delivery, the concentration of hCG falls rapidly and returns to undetectable levels a few days later after. In non-pregnant women or men an increased level of hCG indicates neoplasia. Determining the hCG concentrations can be helpful in monitoring and recognising chorionic tumors or malignancies (such as; pancreatic, testicular and bronchogenic pulmonary cancers).
Reagents which are supplied with the hCG ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: monoclonal anti-beta hCG antibody coated.
2. Standard (Standard 1-5): 5; 50; 200; 500; 1000 mIU/mL.
3. Sample Diluent.
4. Enzyme Conjugate.
5. Substrate Solution.
6. Stop Solution.
Note: Additional Sample Diluent for sample dilution is available upon request.
The minimum sensitivity detection limit of chorionic gonadotropin using current hCG ELISA kit was approximately 5.0 mIU/ml. The dynamic range for this assay is 5.0 – 1,000.0 mIU/ml.
- Immunoassay of human chorionic gonadotropin, its free subunits, and metabolites. Clin Chem. (1997) 43 (12): 2233-43. Cole LA.
- Standardization of Epitopes for Human Chorionic Gonadotropin (hCG) Immunoassays. Curr Med Chem. (2016) 23 (30): 3481-3494. Review. Berger P. and Lapthorn A.J.
- Serum levels of steroid and placental protein hormones in ectopic pregnancy. Eur J Obstet Gynecol Reprod Biol. (1991) 39 (2): 117-22. Mantzavinos T., et al.
- Automated determination of human choriogonadotropin by use of microparticle capture analysis. Clin Chem. (1990) 36 (3): 554-6. Trundle D.S., et al.
- Full Name: Human Chorionic Gonadotropin (hCG) ELISA Kit
- Reactivity: Human
- Sample Type: Serum
- Sensitivity: 5.0 mIU/ml