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Histamine Research ELISA Kit

INTENDED USE

Human histamine research ELISA kit is intended for measuring in vitro quantitative levels of histamine or beta imidazolethylamine in plasma (EDTA) plasma and many other biological sample types. This assay has a minimum detection level of 0.2 ng/ml.

BACKGROUND

Histamine (also known as beta imidazolethylamine) is usually classified as an amine molecule because of its ammonia structure and it mainly stored in the form of granules within the tissues of mast cells. It can also be stored in blood cells which are called basophils that are found to be harbouring histamine-containing granules. Histamine is formed via the removal of a carboxyl group from the histidine molecule and it is found to be present in almost every tissue in the body.

At present there are four histamine receptors that have been identified which are called H1, H2, H3 and H4. Histamine needs to bind to its receptors in order to carry out its specific function and these receptor can be found on the cell surface. It has been linked to number of different functions which include increasing permeability, helping with contraction of smooth muscles, stimulating gastric acid secretion within the stomach, lowering blood pressure and increasing the heart rate. Histamine activity can be inhibited by the use of anti-histamines, these are able to prevent histamines from binding to their receptors and this has be particularly useful in treating allergies and healing peptic ulcers.

HISTAMINE RESEARCH ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Reaction Plate: 1 x 96 well plate, empty.
  • Adhesive Foil.
  • Diluent.
  • Histamine Microtiter Strips: Antigen pre-coated microwell plate.
  • Standards (A-F): Concentrations 0, 0.5, 1.5, 5, 15, 50 ng/ml.
  • Antiserum: Goat anti- histamine antibody.
  • Control 1 + Control 2.
  • Histamine Enzyme Conjugate.
  • TMB Substrate.
  • Wash Buffer (50x Concentrate).
  • Stop Solution
  • Acylation Reagent (Lyophilized).
  • Acylation Diluent.
  • Acylation Buffer.

SENSITIVITY

The minimum sensitivity limit of human beta imidazolethylamine or histamine using current histamine research ELISA kit was approximately 0.2 ng/ml. The dynamic range for this assay is 0.5 – 50 ng/ml.

ASSAY CHARACTERISTICS

– Histamine (Research): ELISA
– Analytical Sensitivity (Limit of Detection, LOD): 0.2 ng/mL (Plasma).
– Analytical Specificity (Cross Reactivity): Histamine (100%), 3-Methyl-Histamine (0.1%), Tyramine (0.01%), Serotonin, Tryptamine, L-Phenylalanine, 5-Hydroxy-Indole-Acetic Acid L-Tyrosine (< 0.001%).

REFERENCES

  1. New molecular targets for treatment of peptic ulcer disease. Drugs. (2003) 63 (17): 1785-97. Review. Lehmann F., et al.
  2. Pharmacological properties of histamine receptor subtypes. Cell Mol Biol (Noisy-le-grand). (1994) 40 (3): 275-81. Review. Arrang J.M.
  3. Mast cells as novel mediators of reproductive processes. Front Immunol. (2013) 4: 29. Woidacki K., et al.
  4. Species-dependent activities of G-protein-coupled receptor ligands: lessons from histamine receptor orthologs. Trends Pharmacol Sci. (2013) 34 (1): 13-32. Review. Strasser A., et al.
  5. Histamine as an adjunct to immunotherapy. Semin Oncol. (2002) 29 (3 Suppl 7): 31-4. Review. Naredi P.

ADDITIONAL INFORMATION

  • Full Name: Histamine Research ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma (EDTA), Other Biological Samples
  • Sensitivity: 0.2 ng/ml

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