GET A QUOTE

Histamine Food ELISA Kit

  • Created on the 10 October, 2017.

BACKGROUND

Histamine is a chemical that is present in all cells of the human body and is also a natural component of many different foods. It is a compound that is involved in a number of processes such as the immune system, central nervous system and proper digestion. Due to the fact that it is able to travel through the bloodstream, histamine is able to affect the brain, gut, lungs, cardiovascular system and skin, this makes it very difficult to pinpoint and diagnose since it is able to contribute to a host of different problems. Histamine is essentially a product of decomposition of histidine that is the result of growth of certain bacteria that are present in seafood. Fish that contain high levels of histamine are found to be linked to a number of examples of poisoning (also referred to as scombroid poisoning). The ELISA assay is designed for measuring quantitative concentrations of histamine derivatives found in food extracts. The procedure uses acylation reagent where histamine has been quantitatively derivatized into N-acylhistamine.

INTENDED USE

Histamine ELISA kit is intended for detecting quantitative amounts of histamine derivatives present in food extracts. This assay has a minimum analytical sensitivity limit of 0.15 ppb.

SENSITIVITY

The minimum detection sensitivity level of histamine derivatives using this histamine ELISA kit was 0.15 ppb. The standard assay range for this kit is 0.0 – 50.0 ppb.

REFERENCES

  1. Synthesis of novel haptens and development of an enzyme-linked immunosorbent assay for quantification of histamine in foods. J Agric Food Chem. (2014) 62 (51): 12299-308. Luo L., et al.
  2. Measurement of histamine in seafood by HPLC, CE, and ELISA: comparison of three techniques. Vet Res Commun. (2005) 29 Suppl 2: 343-6. Muscarella M., et al.
  3. Amperometric biosensor for the determination of histamine in fish samples. FoodChem. (2013) 141 (4): 4066-72. Pérez S., et al.
  4. High-throughput histamine analysis approach in an official control laboratory: analytical methods and four years fish products results. FoodChem. (2014) 153: 437-43. Piersanti A., et al.

ADDITIONAL INFORMATION

  • Full Name: Histamine Food ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.15 ppb

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford