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Histamine ELISA Kit

INTENDED USE

Human histamine ELISA kit is a procedure designed for detecting in vitro quantitative levels of histamine and beta imidazolethylamine in human plasma (EDTA, heparin) and urine samples. This assay has a minimum sensitivity detection limit of 0.12 ng/ml (P) and 0.30 ng/ml (U).

BACKGROUND

Histamine or beta imidazolethylamine, in the human body is found in almost every tissue where it is stored predominately in the form of granules of tissue mast cells. Blood cells which are known as basophils are also harbouring histamine-containing granules. It is formed by the removal of a carboxyl group (decarboxylation) of the amino acid histidine, histamine is essentially classified as an organic amine molecule that is based on the structure of ammonia.

Histamine has many important roles after it has been released from the granules and these include aiding contraction of smooth muscles tissues of the stomach, lungs and uterus; the stimulation of gastric acid secretion within the stomach; acceleration of the hearth rate and increasing permeability whilst lowering blood pressure. It has also been shown to serve as a neurotransmitter where it is able to carry chemical messages between nerve cells. It is able to function through binding to histamine receptors which are located on the surface of specific cells. So far there are four kinds of binding receptors, which are called H1, H2, H3 and H4, however, its activity can also be inhibited by a number of drugs which are called antihistamines and these are able to block histamine from binding to its receptors. Conventional anti histamine have been useful in treating allergies to block H1 receptors (called H1 antagonists) examples include Tagamet (cimetidine) which has the ability to block gastric acid secretion and this has been vital in aiding to heal peptic ulcers.

HISTAMINE ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Adhesive Foil.
  • Wash Buffer (50x Concentrate).
  • Reaction Plate: 1 x 96 well plate, empty.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Stop Solution.
  • Histamine Microtiter Strips.
  • Standards (A-F): Concentrations 0, 0.5, 1.5, 5, 15, 50 ng/ml.
  • Control 1.
  • Control 2.
  • Histamine Antiserum.
  • Acylation Solvent.
  • Acylation Buffer.
  • Acylation Reagent (Lyophilized).

SENSITIVITY

The minimum sensitivity detection limit of human histamine and/or beta imidazolethylamine using current histamine ELISA kit was approximately 0.30 ng/ml (U) and 0.12 ng/ml (P). The dynamic range for this assay is 0.5 – 50 ng/ml.

ASSAY CHARACTERISTICS

– Histamine: ELISA
– Analytical Sensitivity (Limit of Quantitation, LOQ): 0.29 ng/mL (Plasma) and 0.96 ng/mL (Urine).
– Analytical Sensitivity (Limit of Detection, LOD): 0.18 ng/mL (Plasma) and 0.22 ng/mL (Urine).
– Analytical Specificity (Cross Reactivity): Histamine (100%), 3-Methyl-Histamine (0.1%), Tyramine (0.01%), L-Phenylalanine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin (< 0.001%).
– Intra Assay Precision: 13 – 15% (Urine) and 12 – 16% (Plasma).
– Inter Assay Precision: 11 – 14% (Urine) and 8 – 25% (Plasma).
– Linearity: 90 – 124% (Urine) and 85 – 106% (Plasma).
– Recovery: 101 – 119% (Urine) and 78 – 89% (Plasma).

REFERENCES

  1. The role of histamine in neurogenic inflammation. Br J Pharmacol. (2013) 170 (1): 38-45. Review. Rosa A.C. and Fantozzi R.
  2. Developmental roles of brain histamine. Trends Neurosci. (2014) 37 (3): 159-68. Review. Panula P., et al.
  3. Histamine and H1-antihistamines: celebrating a century of progress. J Allergy Clin Immunol. (2011) 128 (6): 1139-1150.e4. Review. Simons F.E. and Simons K.J.
  4. New developments in the use of histamine and histamine receptors. Curr Allergy Asthma Rep. (2011) 11 (2): 94-100. Review. Smuda C. and Bryce P.J.
  5. The neglected role of histamine in Alzheimer’s disease. Am J Alzheimers Dis Other Demen. (2013) 28 (4): 327-36. Review. Naddafi F. and Mirshafiey A.

ADDITIONAL INFORMATION

  • Full Name: Histamine ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma (Heparin, EDTA), Urine
  • Sensitivity: 0.12 ng/ml (P) and 0.30 ng/ml (U)

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