Histamine Cell Culture ELISA Kit

  • Created on the 4 March, 2017.


Histamine (beta-imidazolethylamine) is stored mostly in the form of granules of tissue mast cells (also found in basophils), is present in all tissues in the human body. It can be classified as an amine molecule due to the structure of the ammonia and it is predominately formed by the removal of a carboxyl group from the amino acid histidine.

Histamine can carry out its functions via binding to specific histamine receptors that are found on the surface of cells. Currently, there have been four receptors that have been identified namely H1, H2, H3 and H4. Histamine is associated with a number of vital functions such as stimulating gastric acid secretion in the stomach, increasing the heart rate, helping with contraction of smooth muscles and increasing permeability and lowering blood pressure. Its activity can also be blocked by some specific drugs which are referred to as anti-histamines. These are able to prevent histamine from binding to its receptor thereby preventing them from performing a particular function. The use of anti-histamines is useful in the treatment of allergies and also useful in helping to heal peptic ulcers.


Human histamine culture ELISA kit is a method for detecting in vitro quantitative concentrations of histamine (beta-imidazolethylamine) in human cell culture, urine and whole blood samples. This assay has a minimum detection level of 0.2 ng/ml.


The minimum sensitivity limit of histamine (beta-imidazolethylamine) using this human histamine culture ELISA kit was approximately 0.2 ng/ml. The dynamic assay range for this kit is 0.5 – 50 ng/ml.


  1. Optogenetic probing of fast glutamatergic transmission from hypocretin/orexin to histamine neurons in situ. J Neurosci. (2012) 32 (36): 12437-43. Schöne C., et al.
  2. Histamine production by human neutrophils. FASEB J. (2013) 27 (7): 2902-10. Alcañiz L., et al.
  3. Exploring polyamine regulation by nascent histamine in a human-transfected cell model. Amino Acids. (2010) 38 (2): 561-73. Abrighach H., et al.
  4. Histamine prevents apoptosis in human monocytes. Clin Exp Allergy. (2007) 37 (3): 323-30. Soga F., et al.
  5. Effect of histamine H1 receptor antagonists on TARC/CCL17 and MDC/CCL22 production from CD14+ cells induced by antigenic stimulation in vitro. Int Arch Allergy Immunol. (2011) 155 (1): 38-51. Shoji N., et al.


  • Full Name: Histamine Cell Culture ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture, Whole Blood, Urine
  • Sensitivity: 0.2 ng/ml



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