GET A QUOTE

Histamine Cell Culture ELISA Kit

  • Created on the 4 March, 2017.

BACKGROUND

Histamine (beta-imidazolethylamine) is stored mostly in the form of granules of tissue mast cells (also found in basophils), is present in all tissues in the human body. It can be classified as an amine molecule due to the structure of the ammonia and it is predominately formed by the removal of a carboxyl group from the amino acid histidine.

Histamine can carry out its functions via binding to specific histamine receptors that are found on the surface of cells. Currently, there have been four receptors that have been identified namely H1, H2, H3 and H4. Histamine is associated with a number of vital functions such as stimulating gastric acid secretion in the stomach, increasing the heart rate, helping with contraction of smooth muscles and increasing permeability and lowering blood pressure. Its activity can also be blocked by some specific drugs which are referred to as anti-histamines. These are able to prevent histamine from binding to its receptor thereby preventing them from performing a particular function. The use of anti-histamines is useful in the treatment of allergies and also useful in helping to heal peptic ulcers.

INTENDED USE

Human histamine culture ELISA kit is a method for detecting in vitro quantitative concentrations of histamine (beta-imidazolethylamine) in human cell culture, urine and whole blood samples. This assay has a minimum detection level of 0.2 ng/ml.

SENSITIVITY

The minimum sensitivity limit of histamine (beta-imidazolethylamine) using this human histamine culture ELISA kit was approximately 0.2 ng/ml. The dynamic assay range for this kit is 0.5 – 50 ng/ml.

REFERENCES

  1. Optogenetic probing of fast glutamatergic transmission from hypocretin/orexin to histamine neurons in situ. J Neurosci. (2012) 32 (36): 12437-43. Schöne C., et al.
  2. Histamine production by human neutrophils. FASEB J. (2013) 27 (7): 2902-10. Alcañiz L., et al.
  3. Exploring polyamine regulation by nascent histamine in a human-transfected cell model. Amino Acids. (2010) 38 (2): 561-73. Abrighach H., et al.
  4. Histamine prevents apoptosis in human monocytes. Clin Exp Allergy. (2007) 37 (3): 323-30. Soga F., et al.
  5. Effect of histamine H1 receptor antagonists on TARC/CCL17 and MDC/CCL22 production from CD14+ cells induced by antigenic stimulation in vitro. Int Arch Allergy Immunol. (2011) 155 (1): 38-51. Shoji N., et al.

ADDITIONAL INFORMATION

  • Full Name: Histamine Cell Culture ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture, Whole Blood, Urine
  • Sensitivity: 0.2 ng/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford