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High Sensitive C-Reactive Protein (hs-CRP) ELISA Kit

INTENDED USE

Human hs-CRP ELISA kit is a method for analysing in vitro quantitative high sensitive concentrations c-reactive protein (hs-CRP) in human serum and plasma. This assay has a minimum detection limit of < 0.02 ng/ml.

BACKGROUND

C-reactive protein (CRP) is produced exclusively in the liver and it is interleukin-6 (IL-6) which is the chief mediator for the synthesis of CRP by hepatocytes. It has a total approx. molecular mass of 120.000 Daltons and is made up of a symmetrical pentamer structure. Human CRP is essentially a kind of non-immunoglobulin serum substance which is also a heat labile beta-globulin. It is found to be present in the active stages of many inflammatory disorders such as rheumatoid arthritis, psoriatric arthropathy, Crohn’s disease, systemic lupus erythematosus, Reiter’s syndrome, polyarteritis, ankylosing spondylitis and ulcerative colitis.

The CRP-level can increases dramatically after microbial infections and this property has been particularly helpful for the diagnosis and monitoring a large number of bacterial septicemia in neonates and in many other immune-compromised patients. The concentrations of c-reactive protein begin to rise within 6 hours of an acute inflammatory challenge. Also, many injuries that are responsible for causing necrosis and tissue breakdown are also found to elevate serum CRP levels, examples of these are observed in major surgery, myocardial infarction and thermal burns. A number of widespread malignant diseases including Hodgkin’s disease, lymphosarcoma, non-Hodgkin’s lymphoma and many carcinomas (lung, pancreas, stomach, breast, colon and prostate) can also give rise to high levels of CRP which result from tissue damage that is caused by invading tumour cells.

HS-CRP ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Coated Microtiterstrips: Coated with monoclonal antibodies to human CRP.
  • Standards (A-E): Concentrations 0, 0.4, 1, 5, 10 µg/ml.
  • Enzyme Conjugate.
  • Specimen Dilution Buffer (5x).
  • Washing Solution (20x).
  • TMB Chromogen Solution.
  • Stopping Solution.

SENSITIVITY

The minimum detection sensitivity level of high sensitive human c-reactive protein (hsCRP) using this hs-CRP ELISA kit was 0.02 µg/ml. The dynamic assay range for this kit is 0.4 – 10.0 µg /ml.

ASSAY CHARACTERISTICS

– High Sensitive C-Reactive Protein (hs-CRP): ELISA
– Analytical Sensitivity: 0.02 µg/ml
– CRP values < 1.0 mg/L = Low risk for CVD.
-CRP values 1.0 – 2.9 mg/L = Intermediate risk for CVD
– CRP values > 3.0 mg/L = High risk for CVD
– Intra Assay Precision: 4.1 – 6.9%
– Inter Assay Precision: 5.8 – 6.3%
– Cross-Reactivity: Recognizes natural and recombinant human CRP.

REFERENCES

  1. Atrial fibrillation and CHADS2 risk factors are associated with highly sensitive C-reactive protein incrementally and independently. Pacing Clin Electrophysiol. (2009) 32 (5): 648-52. Crandall M.A., et al.
  2. High sensitivity C-reactive protein in cardiovascular risk assessment. CRP mania or useful screening? Int Angiol. (2003) 22 (1): 15-23. Review. Sellmayer A., et al.
  3. Inflammation in atherothrombosis: how to use high-sensitivity C-reactive protein (hsCRP) in clinical practice. Am Heart Hosp J. (2004) 2 (4 Suppl 1): 4-9. Review. Ridker P.M.
  4. Impact of genetic and environmental factors on hsCRP concentrations and response to therapeutic agents. Clin Chem. (2009) 55 (2): 256-64. Review. Shen J. and Ordovas J.M.

ADDITIONAL INFORMATION

  • Full Name: High Sensitive C-Reactive Protein (hs-CRP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: < 0.02 µg/ml

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