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Heartworm Ag ELISA Kit (Canine)

  • Created on the 2 March, 2018.

BACKGROUND

Heartworms (dirofilaria immitis) are mainly found in dogs, cats and ferrets, they are also known to occur in wild animals for example foxes, wolves and sea lions. Heartworms spend majority of their adult life residing in the right side of the heart and also in the large blood vessels that connect the heart to the lungs. In the heart they produce tiny larvae which are known as microfilariae, these are able to live in the blood stream. When a mosquito sucks the blood of an infected animal, these microfilariae are able to enter the mosquito. After about two weeks these microfilariae are able to develop into larger larvae within the mosquito and then migrate to the mouth of the mosquito. The larvae in now able to enter other animal’s skin when then mosquito bites another animal.

There are many blood tests which can be used in order to detect heartworm infection for example Knott’s test and filter tests. Serologic tests are also available in order to detect antigens of heartworms present in the bloodstream. One of the most common and most frequently used is the ELISA test. There is also evidence that some ELISA antigen tests can cross react to Dirofilaria SPP (rapens/Dipetalonema).

INTENDED USE

Canine heartworm ELISA kit is intended for measuring heartworm antigen (dirofilaria immitis) using canine plasma or serum samples. This assay has a qualitative standard range.

SENSITIVITY

The standard range of canine heartworm (dirofilaria immitis) using this canine heartworm ELISA kit is qualitative. Please contact us for the protocol insert for more details.

REFERENCES

  1. Antibody to the Dirofilaria immitis aspartyl protease inhibitor homologue is a diagnostic marker for feline heartworm infections. J Parasitol. (1998) 84 (6): 1231-6. Frank G.R., et al.
  2. Comparison of a whole-blood agglutination test and an ELISA for the detection of the antigens of Dirofilaria immitis in dogs. Ann Trop Med Parasitol. (1998) 92 (1): 73-7. Wang L.C.
  3. Enzyme immunoassay of Dirofilaria immitis-specific IgE in infected dogs. Vet Parasitol. (1988) 30 (1): 45-56. Gershwin L.J., et al.
  4. Evaluation of a new in-clinic method for the detection of canine heartworm antigen. Vet Parasitol. (2011) 177 (3-4): 387-91. Lee A.C., et al.
  5. Validation for use with coyotes (Canis latrans) of a commercially available enzyme-linked immunosorbent assay for Dirofilaria immitis. Vet Parasitol. (2002) 109 (1-2): 45-58. Sacks B.N., et al.

ADDITIONAL INFORMATION

  • Full Name: Heartworm Ag ELISA Kit (Canine)
  • Reactivity: Canine
  • Sample Type: Serum, Plasma
  • Sensitivity: Qualitative

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford