Rat/Mouse growth hormone ELISA kit can be used for measuring quantitative levels of mouse and rat growth hormone (GH, somatotropin) using plasma or serum samples. This assay has a minimum sensitivity detection limit of < 0.04 ng/ml.
Growth hormone (GH) is also sometimes referred to as somatotropin, it is a 191 amino acid polypeptide which is produced, stored and secreted by somatrotropic cells (anterior pituitary gland). It is a peptide hormone which is responsible for stimulating growth, cell regeneration and cell reproduction in human and many other animals. There are several isoforms of GH which can exist in the pituitary gland and then be released into the blood stream. GH is a stress hormone which functions in elevating the levels of glucose and free fatty acids, it can also stimulate IGF-1 production.
RAT/MOUSE GROWTH HORMONE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: Coated with goat-anti-mouse/rat GH antibodies.
- Standards: Lyophilised, recombinant rat growth hormone.
- Control Serum 1 (Lyophilised).
- Control Serum 2 (Lyophilised).
- Antibody Conjugate.
- Enzyme Conjugate (HRP).
- Dilution Buffer.
- Washing Buffer (20x Concentrated).
- Substrate (TMB).
- Stopping Solution.
- Sealing Tape.
The minimum sensitivity detection limit of rat or mouse growth hormone (GH, somatotropin) using this rat/mouse GH ELISA kit was approximately 0.04 ng/ml. The dynamic range for this assay is 0.15 – 9.0 ng/ml.
– Rat, Mouse Growth Hormone (GH): ELISA
– Analytical Sensitivity: < 0.04 ng/mL.
– Inter-Assay-Variation: 5.48 – 7.41%
– Intra-Assay-Variation: 2.01 – 4.55%
– Species Cross-Reactivity: None (Rabbit, Guinea Pig, Dog, Cat, Chicken, Sheep, Goat, Pig, Donkey, Horse and Bovine).
- Identification of rat growth hormone genomic sequences targeting pituitary expression in transgenic mice. Proc Natl Acad Sci U S A. (1988) 85 (13): 4755-9. Lira S.A., et al.
- Isolation and characterization of rat-mouse somatic cell hybrids secreting growth hormone and prolactin. Exp Cell Res. (1986) 162 (2): 475-85. Melmed S. and Fagin J.A.
- Both estrogen receptor α and β stimulate pituitary GH gene expression. Mol Endocrinol. (2014) 28 (1): 40-52. Avtanski D., et al.
- Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice. Toxicol Sci. (2007) 97 (2): 548-61. Farris G.M., et al.
- Identifying growth hormone-regulated enhancers in the Igf1 locus. Physiol Genomics. (2015) 47 (11): 559-68. Alzhanov D., et al.
- Full Name: Growth Hormone ELISA Kit (Rat/Mouse)
- Reactivity: Rat, Mouse
- Sample Type: Serum, Plasma
- Sensitivity: < 0.04 ng/ml