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Granulocyte Colony-Stimulating Factor (G-CSF) ELISA Kit

  • Created on the 6 February, 2017.

BACKGROUND

Granulocyte colony-stimulating factor (G-CSF or GCSF) is also known as granulocyte colony-stimulating factor 3 (GSF 3), is a hormone-like glycoprotein that is important in regulating hematopoietic cell differentiation and proliferation. A major function of it is to stimulate bone morrow in order to produce stem cells or granulocytes, which are then released into the bloodstream. There is evidence to suggest that G-CSF can stimulate the differentiation, proliferation, survival and function of mature neutrophils and neutrophil precursors. Functionally, it is essentially either a cytokine or a hormone which is be produced by macrophages, endothelium and many other immune cells, existing in two forms 174- and 177-amino acid protein with a total molecular weight of 19,600 grams per mole. A unique property is the effect of G-CSF on myeloid leukemias in order to drive leukemic cells from a self-renewing malignant state into a mature differentiated phenotype, this also results in concomitant loss of tumorigenicity. The pharmaceutical analogs of naturally occurring G-CSF are called lenograstim and filgrastim. The standard used in this ELISA assay is recombinant human G-CSF that consists of 175 amino acids with a total molecular mass of 18.8KDa.

INTENDED USE

Human G-CSF ELISA kit is a reliable quantitative procedure for measuring granulocyte colony-stimulating factor (G-CSF or GCSF) in human serum, plasma (EDTA, heparin, citrate) and cell culture supernatant. This assay has a minimum sensitivity detection limit of < 4.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of granulocyte colony-stimulating factor (G-CSF) using this G-CSF ELISA kit was approximately < 4.0 pg/ml. The dynamic assay range for this kit is 31.2 – 2000.0 pg/ml.

REFERENCES

  1. Human gene coding for granulocyte-colony stimulating factor is assigned to the q21-q22 region of chromosome 17. Somat Cell Mol Genet (1987) 13 (6): 679-84. Kanda N, et al.
  2. G-CSF prevents cardiac remodeling after myocardial infarction by activating the JakStat pathway in cardiomyocytes. Nat Med (2005) 11 (3): 305-11. Harada M, et al.
  3. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature (1986) 319 (6052): 415-8. Nagata S, et al.

ADDITIONAL INFORMATION

  • Full Name: Granulocyte Colony-Stimulating Factor (G-CSF) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (EDTA, Citrate, Heparin), Cell Culture Supernatant
  • Sensitivity: < 4.0 pg/ml

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