G-CSF ELISA Kit (Granulocyte Colony Stimulating Factor)

INTENDED USE

Human G-CSF ELISA kit is a reliable quantitative procedure for measuring granulocyte colony stimulating factor (GCSF, G-CSF) in human biological fluids, serum, plasma and tissue homogenates. This assay has a minimum sensitivity detection limit of 23.438 pg/ml.

BACKGROUND

Granulocyte colony stimulating factor (G-CSF, GCSF) is a hormone-like glycoprotein that is important in regulating hematopoietic cell differentiation and proliferation. A major function of it is to stimulate bone morrow in order to produce stem cells or granulocytes, which are then released into the bloodstream. It can stimulate the differentiation, proliferation, survival and function of mature neutrophils and neutrophil precursors. Functionally, it is essentially either a cytokine or a hormone which is be produced by macrophages, endothelium and many other immune cells, existing in two forms 174- and 177-amino acid protein with a total molecular weight of 19,600 grams per mole.

The standard used in the following assay is recombinant that consists of 175 amino acids with a total molecular mass of 18.8KDa. A unique property is the effect of G-CSF on myeloid leukemias in order to drive leukemic cells from a self-renewing malignant state into a mature differentiated phenotype, this also results in concomitant loss of tumorigenicity. The pharmaceutical analogs of naturally occurring GCSF are called lenograstim and filgrastim.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Standards: Concentrations 0, 39.062, 78.125, 156.25, 312.5, 625, 1250, 2500 pg/ml.
• One 96-Well Plate: Pre-coated with anti G-CSF antibody.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum sensitivity detection limit of granulocyte colony stimulating factor (G-CSF, GCSF) using current G-CSF ELISA kit was approximately 23.438 pg/ml. The dynamic range for this assay is 39.063 – 2500.0 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for GCSF, no cross reactivity or interference between G-CSF and analogues was detected.
– Recovery: Serum (85 – 104%), EDTA Plasma (85 – 101%), Heparin Plasma (86 – 97%).
– Linearity: Serum (86 – 105%), EDTA Plasma (82 – 99%), Heparin Plasma (81 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Human gene coding for granulocyte-colony stimulating factor is assigned to the q21-q22 region of chromosome 17. Somat Cell Mol Genet (1987) 13 (6): 679-84. Kanda N, et al.
2. G-CSF prevents cardiac remodeling after myocardial infarction by activating the JakStat pathway in cardiomyocytes. Nat Med (2005) 11 (3): 305-11. Harada M, et al.
3. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature (1986) 319 (6052): 415-8. Nagata S, et al.

ADDITIONAL INFORMATION

• Full Name: G-CSF ELISA Kit (Granulocyte Colony Stimulating Factor)
• Reactivity: Human
• Sample Type: Serum, Plasma, Tissue Homogenates, Biological Fluids
• Sensitivity: 23.438 pg/ml

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