GET A QUOTE

Glutamic Acid Decarboxylase (Anti-GAD) ELISA Kit

  • Created on the 9 February, 2017.

BACKGROUND

Glutamic acid decarboxylase (anti-GAD) is an essential marker which has been used to serve for the development of autoimmune diabetes present in adults. This has been useful in helping to identify whether an individual has either latent autoimmune diabetes of adulthood (LADA) or type 1 diabetes. It is well known that the onset of autoimmune diabetes is strongly linked with the presence of autoantibody markers, for diagnostic purpose then anti-GAD is the most common. This antibody is found to be present in over 80% of patients that have type 1 diabetes which has developed during childhood or adolescence.
It is been discovered that anti-GAD and many other antibodies which are directed against pancreatic antigens can be detected for many prior to the clinical onset of autoimmune-mediated diabetes, this provides a clear indication that there must be a long “pre-diabetic” phase which is associated with the autoimmune activity. Other antigens which can also be recognised by anti-GAD include glutamic acid decarboxylase (GAD 65kDa isoform), insulin, islet cell antigen IA-2 and ICA-512.

INTENDED USE

Human anti-GAD ELISA kit is a reliable quantitative procedure for measuring glutamic acid decarboxylase (anti-GAD) in human serum. This assay has a minimum sensitivity detection limit of 0.57 U/ml.

SENSITIVITY

The minimum sensitivity detection limit of glutamic acid decarboxylase (anti-GAD) using this anti-GAD ELISA kit was approximately 0.57 U/ml. The dynamic assay range for this kit is 5.0 – 2000.0 U/ml.

REFERENCES

  1. Immunology of stiff person syndrome and other GAD-associated neurological disorders. Expert Rev Clin Immunol. (2013) 9 (11): 1043-53. Review. Alexopoulos H. and Dalakas M.C.
  2. Spectrum of neurological syndromes associated with glutamic acid decarboxylase antibodies: diagnostic clues for this association. Brain. (2008) 131 (Pt 10): 2553-2563. Saiz et al.
  3. The broad clinical phenotype of Type 1 diabetes at presentation. Diabet Med. (2013) 30 (2): 170-8. Merger et al.
  4. Expression of recombinant human glutamic acid decarboxylase (GAD) in myeloma cells and enzyme-linked immunosorbent assay (ELISA) for autoantibodies to GAD. J Biochem. (1997) 121 (1): 20-4. Matsuba T., et al.

ADDITIONAL INFORMATION

  • Full Name: Glutamic Acid Decarboxylase (Anti-GAD) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 0.57 U/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford