Human anti-GAD ELISA kit is a reliable quantitative procedure for measuring anti glutamic acid decarboxylase (anti-GAD, GAD65) in human serum. This assay has a minimum sensitivity detection limit of 0.57 U/ml.
Glutamic acid decarboxylase (GAD) is an essential marker which has been used to serve for the development of autoimmune diabetes present in adults. This has been useful in helping to identify whether an individual has either latent autoimmune diabetes of adulthood (LADA) or type 1 diabetes. It is well known that the onset of autoimmune diabetes is strongly linked with the presence of autoantibody markers, for diagnostic purpose then anti-GAD is the most common. This antibody is found to be present in over 80% of patients that have type 1 diabetes which has developed during childhood or adolescence.
The following human anti-GAD ELISA kit is useful helping to diagnose many different types of medical applications. It is been discovered that anti glutamic acid decarboxylase and many other antibodies which are directed against pancreatic antigens can be detected for many prior to the clinical onset of autoimmune-mediated diabetes, this provides a clear indication that there must be a long “pre-diabetic” phase which is associated with the autoimmune activity. Please note that this anti-GAD ELISA kit is designed to be used only with serum samples. Other antigens which can also be recognised by anti-GAD include anti glutamic acid decarboxylase (GAD 65kDa isoform, GAD65), insulin, islet cell antigen IA-2 and ICA-512.
ANTI-GAD ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Coated Microwells: 12 break-apart strips of 8 wells (96 in total) bound with human anti-GAD antigen.
- Calibrators 1-5: Concentration 0-4000 u/mL (units are NIBSC 97/550), made up from human anti-GAD.
- Control 1 (Positive) and Control 2 (Negative). Containing human anti-GAD antibody.
- Anti-GAD Biotin (Lyophilised).
- Reconstitution Buffer: To be used with anti-GAD biotin.
- Streptavidin Peroxidase (SA-POD)(20x).
- Diluent for SA-POD.
- TMB Peroxidase Substrate.
- Concentrated Wash Solution (10x).
- Stop Solution.
The minimum sensitivity detection limit of anti glutamic acid decarboxylase (anti-GAD, GAD65) using current human anti-GAD ELISA kit was approximately 0.57 U/ml. The dynamic range for this assay is 5.0 – 2000.0 U/ml.
- Immunology of stiff person syndrome and other GAD-associated neurological disorders. Expert Rev Clin Immunol. (2013) 9 (11): 1043-53. Review. Alexopoulos H. and Dalakas M.C.
- Spectrum of neurological syndromes associated with glutamic acid decarboxylase antibodies: diagnostic clues for this association. Brain. (2008) 131 (Pt 10): 2553-2563. Saiz et al.
- The broad clinical phenotype of Type 1 diabetes at presentation. Diabet Med. (2013) 30 (2): 170-8. Merger et al.
- Expression of recombinant human glutamic acid decarboxylase (GAD) in myeloma cells and enzyme-linked immunosorbent assay (ELISA) for autoantibodies to GAD. J Biochem. (1997) 121 (1): 20-4. Matsuba T., et al.
- Full Name: Anti-GAD ELISA Kit
- Reactivity: Human
- Sample Type: Serum
- Sensitivity: 0.57 U/ml