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Gliadin IgM ELISA Kit

  • Created on the 12 June, 2017.

BACKGROUND

Anti-gliadin antibodies (AGA) are made up of three subtypes anti-gliadin IgA (useful in diagnosing coeliac disease patients), anti-gliadin IgG (non-celiac gluten sensitivities and coeliac disease) and anti-gliadin IgE (allergy related conditions). They are present in wheat (as prolamin), existing as 3 alleles AA, BB or DD and are produced in response to gliadin. Each of the alleles are slightly different and therefore able to produce a variety of antibodies. Gliadin are forming crucial components of gluten and become n more widely used in the diagnosis of gluten-induced enteropathy.

INTENDED USE

Human gliadin-IgM ELISA kit can be used for detecting in-vitro quantitative concentrations of human IgM antibodies against gliadin in plasma or serum. This assay has a minimum analytical sensitivity limit of 0.94 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human anti-gliadin IgM using this human gliadin-IgM ELISA kit was 0.94 U/ml. The dynamic assay range for this kit is 1.0 – 300.0 U/ml.

REFERENCES

  1. IgG, IgA and IgE gliadin antibody determinations as screening test for untreated coeliac disease in children, a multicentre study. Eur J Pediatr. (1989) 148 (6): 496-502. Bürgin-Wolff A., et al.
  2. Class and subclass-associated specificity differences of anti-gliadin antibodies from mucosa and serum. Immunology. (1992) 77 (4): 604-8. Engström P.E., et al.
  3. Secretion of gliadin antibody by coeliac jejunal mucosal biopsies cultured in vitro. Clin Exp Immunol. (1986) 64 (1): 119-24. Ciclitira P.J., et al.
  4. Gliadin antibody production by small intestinal lymphocytes from patients with coeliac disease. Int Arch Allergy Appl Immunol. (1989) 89 (2-3): 246-9. Ciclitira P.J., et al.
  5. Immunoreactivity to food antigens in patients with chronic urticaria. Immunol Invest. (2014) 43 (5): 504-16. Rašković S., et al.

ADDITIONAL INFORMATION

  • Full Name: Gliadin IgM ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.94 U/ml

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