Rat GIP active ELISA kit is designed for quantitative analysis of rat active form gastric inhibitory polypeptide (GIP active for, GIP [1-42]) using plasma-EDTA. This assay has a minimum sensitivity detection limit of 0.43 pg/ml.
Gastric inhibitory polypeptide (GIP) is a proprotein which is made up of 153 amino acids, it is a member of the secretin family. One of the primary function of this protein is to stimulate the secretion of insulin, other important roles include inhibiting the secretion of gastric acid and thereby aiding in protecting the small intestines from getting damaged by the stomach acid. There are recent finding indicating that GIP could have critical roles in bone remodelling and the absence of GIP receptors have a direct correlation to resistance to obesity in knockout mice experiments.
RAT GIP ACTIVE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Pre-Coated Plate: Anti-GIP (C) rabbit IgG.
- Labelled Antibody (30x Conc.).
- Standard: Rat GIP (1-42).
- EIA Buffer.
- Solution For Labelled Antibody.
- Chromogen (TMB).
- Stop Solution.
- Wash Buffer (40x Conc.).
The minimum sensitivity detection limit of rat active form gastric inhibitory polypeptide (GIP active, GIP [1-42]) using current rat GIP active ELISA kit was approximately 0.43 pg/ml. The dynamic range for this assay is 2.3 – 150.0 pg/ml.
– Rat GIP Active (1-42): ELISA
– Added Recovery Assay: 83.7 – 97.7%
– Intra – Assay: 5.5 – 7.6%
– Inter – Assay: 5.3 – 8.8%
– Specificity: Rat GIP (1-42)( 100%)
– Sensitivity: 0.43 pg/mL
- Release of gastric inhibitory polypeptide (GIP) by intraduodenal acidification in rats and humans and abolishment of the incretin effect of acid by GIP-antiserum in rats. Gastroenterology. (1979) 76 (3): 515-23. Ebert R., et al.
- Fructose stimulates GLP-1 but not GIP secretion in mice, rats, and humans. Am J Physiol Gastrointest Liver Physiol. (2014) 306 (7): G622-30. Kuhre R.E., et al.
- Gastric inhibitory polypeptide (GIP) binding sites in rat brain. Peptides. (1994) 15 (2): 297-302. Kaplan A.M. and Vigna S.R.
- Dietary 1-monoolein decreases postprandial GIP release by reducing jejunal transport of glucose and fatty acid in rodents. Am J Physiol Gastrointest Liver Physiol. (2012) 303 (3): G298-310. Shimotoyodome A., et al.
- Full Name: GIP Active Form ELISA Kit (Rat)
- Reactivity: Rat
- Sample Type: EDTA-Plasma
- Sensitivity: 0.43 pg/ml