Rat GIP Active ELISA Kit (Gastric Inhibitory Polypeptide)

Full Name: Rat GIP Active ELISA Kit (Gastric Inhibitory Polypeptide)
Reactivity: Rat
Sample Type: EDTA-Plasma
Sensitivity: 0.43 pg/ml

BACKGROUND

The 153-amino acid proprotein known as gastric inhibitory polypeptide (GIP) belongs to the secretin family. Endocrine cells in the small intestine produce GIP, which is then released into the bloodstream. It acts as an orexigenic hormone, stimulating hunger and promoting fat storage. iIt has also been shown to have a role in blood glucose control. The stomach inhibitory peptide family is made up of the peptide hormones bombesin and gastric inhibitory peptide (GIP). These are members of the secretin family, which includes the well-known hormone, ghrelin. Enteroendocrine cells mostly found in the duodenum, jejunum, and ileum create GIPs. They are known to be important in appetite regulation.

GIP suppresses gastric acid production, which aids in avoiding stomach acid harm to the small intestines. There are recent finding indicating that GIP could have critical roles in bone remodelling and the absence of GIP-receptors have a direct correlation to resistance to obesity in knockout mice experiments. It has also been identified as a potential regulator of bone maturation, as its expression is regulated by the GH/IGF-1 axis. GIP knockouts are less responsive to the growth hormone (GH) and IGF-1 axis, which increases the risk for obesity.

INTENDED USE

Rat GIP active ELISA kit is designed for quantitative analysis of gastric inhibitory polypeptide (GIP active for, GIP [1-42]) using plasma-EDTA. This assay has a minimum sensitivity detection limit of 0.43 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Pre-Coated Plate: Anti-GIP (C) rabbit IgG.
• Labelled Antibody (30x Conc.).
• Standard: Rat GIP (1-42).
• EIA Buffer.
• Solution For Labelled Antibody.
• Chromogen (TMB).
• Stop Solution.
• Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of gastric inhibitory polypeptide (GIP active, GIP [1-42]) using current rat GIP active ELISA kit was approximately 0.43 pg/ml. The dynamic range for this assay is 2.3 – 150.0 pg/ml.

ASSAY CHARACTERISTICS

– Added Recovery Assay: 83.7 – 97.7%
– Intra – Assay: 5.5 – 7.6%
– Inter – Assay: 5.3 – 8.8%
– Specificity: Rat GIP (1-42)( 100%)
– Sensitivity: 0.43 pg/mL

REFERENCES

1. Release of gastric inhibitory polypeptide (GIP) by intraduodenal acidification in rats and humans and abolishment of the incretin effect of acid by GIP-antiserum in rats. Gastroenterology. (1979) 76 (3): 515-23. Ebert R., et al.
2. Fructose stimulates GLP-1 but not GIP secretion in mice, rats, and humans. Am J Physiol Gastrointest Liver Physiol. (2014) 306 (7): G622-30. Kuhre R.E., et al.
3. Gastric inhibitory polypeptide (GIP) binding sites in rat brain. Peptides. (1994) 15 (2): 297-302. Kaplan A.M. and Vigna S.R.
4. Dietary 1-monoolein decreases postprandial GIP release by reducing jejunal transport of glucose and fatty acid in rodents. Am J Physiol Gastrointest Liver Physiol. (2012) 303 (3): G298-310. Shimotoyodome A., et al.

OTHER RELATED ELISA KITS

• GIP Active Form ELISA Kit (Mouse)
• Leptin ELISA Kit (Rat/Mouse)
• Insulin ELISA Kit (Rat)