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Gamma Aminobutyric Acid (GABA) ELISA Kit

INTENDED USE

Human GABA ELISA kit is designed for determining in vitro quantitative concentrations of gamma aminobutyric acid (GABA) in human plasma, serum and urine samples. This assay has a minimum sensitivity detection limit of 49 ng/ml.

BACKGROUND

GABA (gamma aminobutyric acid) is an inhibitory neurotransmitter, which functions to slow down or weaken neuron signals. This is unlike other neurotransmitters (adrenaline, nor-adrenaline, serotonin, dopamine and glutamate) which all have an excitatory function (i.e. they are responsible for stimulating neuron firing). Gamma aminobutyric acid occurs naturally within the brain where it is vital in regulating the brain activity, there is also evidence to supports its important role in regulating muscle tone. GABA is technically an amino acid, even though it is not part of any protein either in your body or the food you eat. Very small amounts of it can be obtained from food, however, the majority of it is synthesised from glutamate in the body. This human GABA ELISA kit is designed to be used for analysing serum, urine and plasma samples.

Due to its inhibitory function, it plays a vital plays role in anxiety. In this situation, when nerve signals fire too quickly and possess anxiety-inducing signals, GABA is activated and acts in order to slow the signals down and therefore reducing overwhelming feelings of anxiety. However, in individuals with anxiety disorders (e.g. post traumatic stress, panic disorder and generalised anxiety), gamma aminobutyric acid does not function as it should and this results in elevated levels of anxiety. This assay is intended to be used for a wide range of diagnostic applications. There are many remedies and medications which are currently being used for anxiety, benzodiazepines (valium and xanax) are able reduce anxiety through the way they interact with GABA receptors. Whereas, coffee can inhibit GABA and therefore make an individual more buzzed up or anxious.

GABA ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Adhesive Foil.
  • Microtiter Plate: 2 x 48 well plate, empty.
  • Extraction Plate – 2 x 48 well plate, pre-coated with cation exchanger.
  • Wash Buffer Concentrate (50x).
  • Enzyme Conjugate.
  • Substrate (TMB).
  • Stop Solution.
  • GABA Microtiter Strips: Antigen pre-coated microwell plate.
  • Antiserum: Rabbit anti-GABA antibody.
  • Standards (A-F): Concentrations 0, 75, 250, 750, 2,500, 7,500 ng/ml.
  • Control 1.
  • Control 2.
  • Assay Buffer.
  • Equalizing Reagent.
  • D-Reagent: Crosslinking agent in dimethylsulfoxide.
  • Q-Buffer.
  • I-Buffer (concentrated).
  • Elution-Buffer.
  • Diluent.
  • NaOH Solution.

SENSITIVITY

The minimum sensitivity detection limit of GABA (gamma aminobutyric acid) using current human GABA ELISA kit was approximately 49 ng/ml. The dynamic range for this assay is 75 – 7,500 ng/ml.

ASSAY CHARACTERISTICS

– Gamma-Aminobutyric Acid (GABA): ELISA
– Analytical Specificity (Cross Reactivity): GABA (100%), ß-Alanine (0.50%), α-Aminobutyric acid (< 0.001%).
– Intra Assay Precision: 10.0 – 11.0%
– Inter Assay Precision: 14.0 – 16.0%
– Recovery: 104 – 111% (Male) and 105 – 118% (Female).

REFERENCES

  1. Prefrontal cortical gamma-aminobutyric acid transmission and cognitive function: drawing links to schizophrenia from preclinical research. Biol Psychiatry. (2015) 77 (11): 929-39. Review. Tse M.T., et al.
  2. Molecular aspects of age-related cognitive decline: the role of GABA signaling. Trends Mol Med. (2015) 21 (7): 450-60. Review. McQuail J.A., et al.
  3. Brain γ-aminobutyric acid: a neglected role in impulsivity. Eur J Neurosci. (2014) 39 (11): 1921-32. Review. Hayes D.J., et al.
  4. Neurogenesis mediated by gamma-aminobutyric acid and glutamate signaling. J Pharmacol Sci. (2009) 110 (2): 133-49. Review. Nakamichi N., et al.
  5. Hypoxia and GABA shunt activation in the pathogenesis of Alzheimer’s disease. Neurochem Int. (2016) 92: 13-24. Review. Salminen A., et al.

ADDITIONAL INFORMATION

  • Full Name: Gamma Aminobutyric Acid (GABA) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Urine
  • Sensitivity: 49 ng/ml

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