GABA ELISA Kit (Gamma-Aminobutyric Acid)

INTENDED USE

Human GABA ELISA kit is designed for determining in vitro quantitative concentrations of gamma aminobutyric acid (GABA) in human plasma, serum and urine samples. This assay has a minimum sensitivity detection limit of 49 ng/ml.

BACKGROUND

Gamma aminobutyric acid (GABA) is an inhibitory neurotransmitter, which functions to slow down or weaken neuron signals. It occurs naturally within the brain where it is vital in regulating the brain activity, there is also evidence to supports its important role in regulating muscle tone. Very small amounts are obtained from food, however, the majority of it is synthesised from glutamate in the body.

Due to its inhibitory function, GABA plays a vital plays role in anxiety. In this situation, when nerve signals fire too quickly and possess anxiety-inducing signals, it is activated and acts in order to slow the signals down and therefore reducing overwhelming feelings of anxiety. In individuals with anxiety disorders (e.g. post traumatic stress, panic disorder and generalised anxiety), it does not function as it should and this results in elevated levels of anxiety.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Adhesive Foil.
• Microtiter Plate: 2 x 48 well plate, empty.
• Extraction Plate – 2 x 48 well plate, pre-coated with cation exchanger.
• Wash Buffer Concentrate (50x).
• Enzyme Conjugate.
• Substrate (TMB).
• Stop Solution.
• GABA Microtiter Strips: Antigen pre-coated microwell plate.
• Antiserum: Rabbit anti-GABA antibody.
• Standards (A-F): Concentrations 0, 75, 250, 750, 2,500, 7,500 ng/ml.
• Control 1.
• Control 2.
• Assay Buffer.
• Equalizing Reagent.
• D-Reagent: Crosslinking agent in dimethylsulfoxide.
• Q-Buffer.
• I-Buffer (concentrated).
• Elution-Buffer.
• Diluent.
• NaOH Solution.

SENSITIVITY

The minimum sensitivity detection limit of gamma aminobutyric acid (GABA) using current GABA ELISA kit was approximately 49 ng/ml. The dynamic range for this assay is 75 – 7,500 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Specificity (Cross Reactivity): GABA (100%), ß-Alanine (0.50%), α-Aminobutyric acid (< 0.001%).
– Intra Assay Precision: 10.0 – 11.0%
– Inter Assay Precision: 14.0 – 16.0%
– Recovery: 104 – 111% (Male) and 105 – 118% (Female).

REFERENCES

1. Prefrontal cortical gamma-aminobutyric acid transmission and cognitive function: drawing links to schizophrenia from preclinical research. Biol Psychiatry. (2015) 77 (11): 929-39. Review. Tse M.T., et al.
2. Molecular aspects of age-related cognitive decline: the role of GABA-signaling. Trends Mol Med. (2015) 21 (7): 450-60. Review. McQuail J.A., et al.
3. Brain γ-aminobutyric acid: a neglected role in impulsivity. Eur J Neurosci. (2014) 39 (11): 1921-32. Review. Hayes D.J., et al.
4. Hypoxia and GABA shunt activation in the pathogenesis of Alzheimer’s disease. Neurochem Int. (2016) 92: 13-24. Review. Salminen A., et al.

ADDITIONAL INFORMATION

• Full Name: GABA ELISA Kit (Gamma-Aminobutyric Acid)
• Reactivity: Human
• Sample Type: Serum, Plasma, Urine
• Sensitivity: 49 ng/ml

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