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Fumonisin ELISA Kit

  • Created on the 10 October, 2017.

BACKGROUND

Fumonisins belong to a group of mycotoxins which are derived from Fusarium, Liseola section (a common grain mold). There are usually more specifically referred to as Fumonisin B1, B2 or B3. These toxins are extreme stability to high temperatures (reaching up to 100°C), and they can also remain active within contaminated food for many years. Fumonisin can be found in many different types of grains including oats and maize. In human patients there is evidence to indicate the appearance of esophagus cancer which has been linked to exposition to fumonisin B1 and when ingested by animals, fumonisin can result in hepatotoxicity, lung edema and neurotoxicity (mainly identified in pigs and horses). The ELISA assay provides a highly sensitive procedure for analysing rapid quantification of fumonisin contaminations that may be present in cereals or beer.

INTENDED USE

Fumonisn ELISA kit is intended for detecting rapid quantitative amounts of fumonisin present in beer/gyle or cereals. This assay has a minimum analytical sensitivity limit of 0.015 ppm.

SENSITIVITY

The minimum detection sensitivity level of fumonisin using this fumonisin ELISA kit was 0.015 ppm. The standard assay range for this kit is 0.05 – 5.0 ppm.

REFERENCES

  1. Application of mimotope peptides of fumonisin b1 in Peptide ELISA. J Agric Food Chem. (2013) 61 (20): 4765-70. Liu X., et al.
  2. Development of a single-chain variable fragment antibody-based enzyme-linked immunosorbent assay for determination of fumonisin B₁ in corn samples. J Sci Food Agric. (2014) 94 (9): 1865-71. Zou L., et al.
  3. Correlation of deoxynivalenol and fumonisin concentration determined in maize by ELISA methods. J Immunoassay Immunochem. (2012) 33 (4): 414-21. Pleadin J., et al.
  4. Development of an electrochemical immunosensor for fumonisins detection in foods. Toxins (Basel). (2010) 2 (4): 382-98. Kadir M.K. and Tothill I.E.
  5. Stability of fumonisins in thermally processed corn products. J Food Prot. (1998) 61 (8): 1030-3. Castelo M.M., et al.

ADDITIONAL INFORMATION

  • Full Name: Fumonisn ELISA Kit
  • Sample Type: Cereal and Beer
  • Sensitivity: 0.015 ppm

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