GET A QUOTE

Free Testosterone Saliva ELISA Kit

  • Created on the 23 February, 2017.

BACKGROUND

Testosterone is a C19 steroid hormone that is mainly produced in the leydig cells of the testes of males and in smaller quantities formed in the ovaries of females. It is known to play vital functions during the development of the male reproductive tissues, bone mass, hair growth, sexual function, supporting increased muscles, stamina, cardiovascular health and the general well-being of the immune system. The determination of testosterone has been useful in diagnosing and treating disorders that involve androgens. These include primary or secondary hypogonadism, disorders originating from testicular problems, defects of the hypothalamus and pituitary glands. Other disorders are impotence in males, virilization (masculinization) due to tumors, hirsutism (excessive hair) in females, adrenogenital syndroms and polycystic ovaries.

In both males and females a large quantity of testosterone is produced from the conversion of circulating androstenedione, DHEA and DHEA-S. It also displays a diurnal rhythm where the highest levels are seen in the morning and the lowest around midnight. Generally adult males usually have around 10-20 fold higher testosterone plasma concentrations present when compared to females. During circulation it is found that the main part of testosterone is bound to plasma proteins for example sex hormone binding globulin (SHBG) and albumin. Only a small portion approx. 1-2 % of the testosterone is unbound or free, this represents the biologically active form. In the cells a large quantity of this hormone can be converted to 5a-dihydrotestosterone, the free testosterone can also be released via the salivary glands. There is a direct correlation between the level of testosterone in saliva and the level of free testosterone present in plasma.

So far, every attempts for a direct quantification of free testosterone using either serum or plasma samples with a commercial immunoassays has failed. The only reliable alternative is using salivary samples to measure it, there have been many publication which have proved that the analysis of free testosterone using saliva provides clinically valid results even when the concentrations being measured are quite low. Also, using salivary methods it is easier to compensate for the episodic secretion pattern by carrying out multiple sampling over a given period of time. A mixture of these sample can then be used to carry out the analysis procedure, whereas only using single salvia sample per analysis will give arbitrary results, similar to those obtained for serum and plasma samples.

INTENDED USE

Free testosterone in saliva ELISA kit is an accurate in vitro quantitative method for detecting free testosterone (17beta-hydroxy-4-androstene-3-one) in human saliva samples. This assay has a minimum sensitivity detection limit of 2.2 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of testosterone using this human free testosterone in saliva ELISA kit was approximately 2.2 pg/ml. The dynamic assay range for this kit is 10.0 – 1,000.0 pg/ml.

REFERENCES

  1. Assessing biological maturity: chronological age and the pubertal development scale predict free testosterone in adolescent males. J Pediatr Endocrinol Metab. (2015) 28 (3-4): 381-6. Hibberd E.E., et al.
  2. Analytical Evaluation of Free Testosterone and Cortisol Immunoassays in Saliva as a Reliable Alternative to Serum in Sports Medicine. J Clin Lab Anal. (2016) 30 (5): 732-5. Lippi G., et al.
  3. Relationships between salivary free testosterone and the expression of force and power in elite athletes. J Sports Med Phys Fitness. (2012) 52 (2): 221-7. Crewther B.T., et al.
  4. A critical evaluation of salivary testosterone as a method for the assessment of serum testosterone. Steroids. (2014) 86: 5-9. Fiers T., et al.

ADDITIONAL INFORMATION

  • Full Name: Free Testosterone Saliva ELISA Kit
  • Reactivity: Human
  • Sample Type: Saliva
  • Sensitivity: 2.2 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford